Immunosensors for atrazine detection in red wine samples

Enrique Valera, Ángel Rodríguez, Javier Ramón-Azcón, Francisco J. Sanchez, M. Pilar Marco

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    Two novel immunosensors, one impedimetric and other one conductimetric, for atrazine detection in red wine samples have been developed. Impedimetric immunosensor is based on an array of interdigitated \i-electrodes (IDnEs) and bioreagents specifically developed to detect this pesticide. Conductimetric immunosensor incorporates additionally gold nanoparticles. Bioreagents were covalently immobilized on the surface of the electrodes (interdigital space). In both cases the biochemical determination of atrazine is possible without any redox mediator. For the case of the impedimetric immunosensor, the detection method is based on impedimetric measurements (in a wide range of frequencies), whereas in the case of the conductimetric immunosensor the detection method is based on conductimetric measurements (DC measurements).The potential of the impedimetric immunosensor to analyze atrazine in complex sample matrices, such as red wine, have been evaluated. This immunosensor can detect atrazine with limits of detection in the order sub-ppb, far below the maximum residue level (MRL) (50 \ig L"1) established by Euro ean Union (EU) for residues of this herbicide in the wine grapes.

    Original languageEnglish
    Title of host publicationBIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices
    Pages226-230
    Number of pages5
    Publication statusPublished - 2009 Jul 21
    Event2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009 - Porto, Portugal
    Duration: 2009 Jan 142009 Jan 17

    Publication series

    NameBIODEVICES 2009 - Proceedings of the 2nd International Conference on Biomedical Electronics and Devices

    Other

    Other2nd International Conference on Biomedical Electronics and Devices, BIODEVICES 2009
    CountryPortugal
    CityPorto
    Period09/1/1409/1/17

    Keywords

    • Atrazine
    • Conductive measurements
    • Food safety
    • Immunosensor
    • Impedance spectroscopy
    • Interdigitated μ-electrodes
    • Wine matrix effect

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Electrical and Electronic Engineering

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