Immunoreactive C-type natriuretic peptide in human adrenal glands and adrenal tumors

Kazuhito Totsune, Kazuhiro Takahashi, Osamu Murakami, Keiichi Itoi, Masahiko Sone, Makoto Ohneda, Fumitoshi Satoh, Yukio Miura, Toraichi Mouri

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

C-type natriuretic peptide (CNP) in human adrenal glands and adrenal tumors was measured with a specific radioimmunoassay for CNP. Tissue immunoreactive (IR-) CNP concentrations were 0.54±0.40 pmol/g wet tissue (gwt) (mean ± SD) in 14 pheochromocytomas, 0.69 ± 0.19 pmol/gwt in six adrenocortical tumors, and 0.49 ± 0.22 pmol/gwt in seven normal adrenal glands (cortex and medulla mixed). These concentrations were comparable to those found in tissues from human brains. Sephadex G-50 superfine column chromatography and reverse-phase high performance liquid chromatography revealed that IR-CNP in normal adrenal glands and pheochromocytoma consisted of at least two components: a component in low molecular weight form chromatographically identical to CNP-22 and the other, a high molecular weight form very similar to human CNP-53. This study has shown that IR-CNP is present in human adrenal glands and adrenal tumors with similar molecular forms and comparable concentrations to those in the human brain.

Original languageEnglish
Pages (from-to)287-290
Number of pages4
JournalPeptides
Volume15
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • Adrenal gland
  • C-type natriuretic peptide
  • Gel chromatography
  • High performance liquid chromatography
  • Pheochromocytoma
  • Primary aldosteronism
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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