TY - JOUR
T1 - Immunological evidence that the β isoform of Ca2+/calmodulin-dependent protein kinase IV is a cerebellar granule cell-specific product of the CaM kinase IV gene
AU - Sakagami, H.
AU - Umemiya, M.
AU - Kobayashi, T.
AU - Saito, S.
AU - Kondo, H.
PY - 1999
Y1 - 1999
N2 - Ca2+/calmodulin-dependent protein kinase IV (CaM kinase IV) exists as two monomeric isoforms, α and β. In this study, we raised an antibody against the β isoform and provided immunohistochemical evidence for specific expression of the β isoform in cerebellar granule cells as a single gene-derived translational product distinct from the α isoform. Immunohistochemical examination showed that the β-immunoreactivity was confined to the nuclei of the cerebellar granule cells, in contrast to the more widespread immunoreactivity for the α isoform in both nuclei and cytoplasm of the cerebellar granule cells and many other neurons with dominant nuclear localization. In developing cerebella, the β-immunoreactivity gradually appeared in the internal granule cells during the postnatal 2nd and 3rd weeks, while the α-immunoreactivity had already appeared in the internal granule cells in the 1st postnatal week. Unlike the α isoform, β-immunoreactivity was not detected in the Purkinje cells at any developmental stages. The differential expression of the α and β isoforms suggests that each isoform may be involved in different cerebellar functions.
AB - Ca2+/calmodulin-dependent protein kinase IV (CaM kinase IV) exists as two monomeric isoforms, α and β. In this study, we raised an antibody against the β isoform and provided immunohistochemical evidence for specific expression of the β isoform in cerebellar granule cells as a single gene-derived translational product distinct from the α isoform. Immunohistochemical examination showed that the β-immunoreactivity was confined to the nuclei of the cerebellar granule cells, in contrast to the more widespread immunoreactivity for the α isoform in both nuclei and cytoplasm of the cerebellar granule cells and many other neurons with dominant nuclear localization. In developing cerebella, the β-immunoreactivity gradually appeared in the internal granule cells during the postnatal 2nd and 3rd weeks, while the α-immunoreactivity had already appeared in the internal granule cells in the 1st postnatal week. Unlike the α isoform, β-immunoreactivity was not detected in the Purkinje cells at any developmental stages. The differential expression of the α and β isoforms suggests that each isoform may be involved in different cerebellar functions.
KW - Ca/calmodulin-dependent protein kinase
KW - Cerebellum
KW - Immunohistochemistry
KW - Nucleus
KW - Rat
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U2 - 10.1046/j.1460-9568.1999.00675.x
DO - 10.1046/j.1460-9568.1999.00675.x
M3 - Article
C2 - 10383642
AN - SCOPUS:0032969284
VL - 11
SP - 2531
EP - 2536
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
SN - 0953-816X
IS - 7
ER -