Immunological evidence that the β isoform of Ca2+/calmodulin-dependent protein kinase IV is a cerebellar granule cell-specific product of the CaM kinase IV gene

H. Sakagami, M. Umemiya, T. Kobayashi, S. Saito, H. Kondo

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Ca2+/calmodulin-dependent protein kinase IV (CaM kinase IV) exists as two monomeric isoforms, α and β. In this study, we raised an antibody against the β isoform and provided immunohistochemical evidence for specific expression of the β isoform in cerebellar granule cells as a single gene-derived translational product distinct from the α isoform. Immunohistochemical examination showed that the β-immunoreactivity was confined to the nuclei of the cerebellar granule cells, in contrast to the more widespread immunoreactivity for the α isoform in both nuclei and cytoplasm of the cerebellar granule cells and many other neurons with dominant nuclear localization. In developing cerebella, the β-immunoreactivity gradually appeared in the internal granule cells during the postnatal 2nd and 3rd weeks, while the α-immunoreactivity had already appeared in the internal granule cells in the 1st postnatal week. Unlike the α isoform, β-immunoreactivity was not detected in the Purkinje cells at any developmental stages. The differential expression of the α and β isoforms suggests that each isoform may be involved in different cerebellar functions.

Original languageEnglish
Pages (from-to)2531-2536
Number of pages6
JournalEuropean Journal of Neuroscience
Volume11
Issue number7
DOIs
Publication statusPublished - 1999

Keywords

  • Ca/calmodulin-dependent protein kinase
  • Cerebellum
  • Immunohistochemistry
  • Nucleus
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

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