Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees

Miyoshi Haruta, Masatsune Murata, Hiroshi Kadokura, Seiichi Homma

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.

Original languageEnglish
Pages (from-to)1021-1025
Number of pages5
JournalPhytochemistry
Volume50
Issue number6
DOIs
Publication statusPublished - 1999 Mar 20
Externally publishedYes

Keywords

  • Enzymatic browning
  • Eriobotrya japonica (Japanese loquat)
  • Polyphenol oxidase
  • Prunus persica (peach)
  • Pseudocydonia sinensis (Chinese quince)
  • Pyrus communis (pear)
  • Pyrus pyrifolia (Japanese pear)
  • Rosaceae

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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