Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells

Toyoaki Anai, Evalour T. Aspuria, Nobuharu Fujii, Takashi Ueda, Minami Matsui, Koji Hasegawa, Hirofumi Uchimiya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

ara genes encoding YPT/RAB like small GTP-binding proteins, which are involved in the vesicular transport system in various eukaryotic cells, have been isolated from Arabidopsis thaliana. To determine the subcellular localization of ARA-4 protein, suspensions of cultured transgenic tobacco cells were analyzed using monoclonal antibody to Arabidopsis thaliana ARA-4 protein. ARA-4 protein was found in the microsomal membrane fraction. Similar results were obtained withArabidopsis thaliana cells. The ARA-4 protein was readily released by detergents, such as 0.5 % sodium deoxycholate and NP-40. The results of immuno-blot analysis with extracts of different organs show that the ARA-4 protein is present in most organs of Arabidopsis thaliana plants, e. g. root, stem, flower and young fruit, at the same level in all organs except for root, where the level is lower.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalJournal of Plant Physiology
Volume147
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • 5-bromo-4-chloro-3-indolyl phosphate
  • A rabidopsis thaliana
  • BCIP
  • CBB
  • GST
  • IPTG
  • NBT
  • Nicotiana tabacum
  • PVDF
  • SDS-PAGE
  • YPT/RAB
  • coomassie brilliant blue
  • glutathione S-transferase
  • isopropyl-β-D-thiogalactopyranoside
  • monoclonal antibody
  • nitro blue tetrazolium
  • polyvinylidene difluoride
  • small GTP-binding protein
  • sodium dodecyl sulfate polyacrylamide gel electrophoresis
  • transgenic plant

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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