Immunolocalization of aromatase, 17α-hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

H. Sasano; M. Okamoto; J. I. Mason; E. R. Simpson; C. R. Mendelson; N. Sasano; S. G. Silverberg

Immunolocalization of aromatase, 17α-hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

H. Sasano, M. Okamoto, J. I. Mason, E. R. Simpson, C. R. Mendelson, N. Sasano, S. G. Silverberg

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

176 Citations (Scopus)

Abstract

Immunohistochemical localization of cholesterol side-chain-cleavage, 17α-hydroxylase and aromatase cytochromes P-450 was performed in 35 morphologically normal human premenopausal ovaries by using specific antibodies against the enzymes. In well-developed ovarian follicles in the late stages of follicular growth, immunoreactivity of P-450(AROM) was only seen in granulosa cells while P-450(17α) and P-450(SCC) activity was confined to theca interna cells, confirming that follicular oestrogen is produced in granulosa cells by the aromatization of androgens derived from the theca interna cells. In the corpus luteum, this functional differentiation is maintained, since immunoreactivity of P-450(AROM) was exclusively present in luteinized granulosa cells while that of P-450(17α) was present in luteinized theca cells. Immunoreactivity of P-450(SCC) was present in both types of cells in the corpus luteum.

Original language	English
Pages (from-to)	163-169
Number of pages	7
Journal	Journal of Reproduction and Fertility
Volume	85
Issue number	1
Publication status	Published - 1989

ASJC Scopus subject areas

Physiology
Embryology
Molecular Biology
Obstetrics and Gynaecology
Developmental Biology

Cite this

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title = "Immunolocalization of aromatase, 17α-hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary",

abstract = "Immunohistochemical localization of cholesterol side-chain-cleavage, 17α-hydroxylase and aromatase cytochromes P-450 was performed in 35 morphologically normal human premenopausal ovaries by using specific antibodies against the enzymes. In well-developed ovarian follicles in the late stages of follicular growth, immunoreactivity of P-450(AROM) was only seen in granulosa cells while P-450(17α) and P-450(SCC) activity was confined to theca interna cells, confirming that follicular oestrogen is produced in granulosa cells by the aromatization of androgens derived from the theca interna cells. In the corpus luteum, this functional differentiation is maintained, since immunoreactivity of P-450(AROM) was exclusively present in luteinized granulosa cells while that of P-450(17α) was present in luteinized theca cells. Immunoreactivity of P-450(SCC) was present in both types of cells in the corpus luteum.",

author = "H. Sasano and M. Okamoto and Mason, {J. I.} and Simpson, {E. R.} and Mendelson, {C. R.} and N. Sasano and Silverberg, {S. G.}",

year = "1989",

language = "English",

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pages = "163--169",

journal = "Journal of Reproduction and Fertility",

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T1 - Immunolocalization of aromatase, 17α-hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

AU - Sasano, H.

AU - Okamoto, M.

AU - Mason, J. I.

AU - Simpson, E. R.

AU - Mendelson, C. R.

AU - Sasano, N.

AU - Silverberg, S. G.

PY - 1989

Y1 - 1989

N2 - Immunohistochemical localization of cholesterol side-chain-cleavage, 17α-hydroxylase and aromatase cytochromes P-450 was performed in 35 morphologically normal human premenopausal ovaries by using specific antibodies against the enzymes. In well-developed ovarian follicles in the late stages of follicular growth, immunoreactivity of P-450(AROM) was only seen in granulosa cells while P-450(17α) and P-450(SCC) activity was confined to theca interna cells, confirming that follicular oestrogen is produced in granulosa cells by the aromatization of androgens derived from the theca interna cells. In the corpus luteum, this functional differentiation is maintained, since immunoreactivity of P-450(AROM) was exclusively present in luteinized granulosa cells while that of P-450(17α) was present in luteinized theca cells. Immunoreactivity of P-450(SCC) was present in both types of cells in the corpus luteum.

AB - Immunohistochemical localization of cholesterol side-chain-cleavage, 17α-hydroxylase and aromatase cytochromes P-450 was performed in 35 morphologically normal human premenopausal ovaries by using specific antibodies against the enzymes. In well-developed ovarian follicles in the late stages of follicular growth, immunoreactivity of P-450(AROM) was only seen in granulosa cells while P-450(17α) and P-450(SCC) activity was confined to theca interna cells, confirming that follicular oestrogen is produced in granulosa cells by the aromatization of androgens derived from the theca interna cells. In the corpus luteum, this functional differentiation is maintained, since immunoreactivity of P-450(AROM) was exclusively present in luteinized granulosa cells while that of P-450(17α) was present in luteinized theca cells. Immunoreactivity of P-450(SCC) was present in both types of cells in the corpus luteum.

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AN - SCOPUS:0024599859

SN - 0022-4251

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JO - Journal of Reproduction and Fertility

JF - Journal of Reproduction and Fertility

IS - 1

ER -

Immunolocalization of aromatase, 17α-hydroxylase and side-chain-cleavage cytochromes P-450 in the human ovary

Abstract

ASJC Scopus subject areas

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