Immunohistochemistry on IDH 1/2, ATRX, p53 and Ki-67 substitute molecular genetic testing and predict patient prognosis in grade III adult diffuse gliomas

Shingo Takano, Eiichi Ishikawa, Noriaki Sakamoto, Masahide Matsuda, Hiroyoshi Akutsu, Masayuki Noguchi, Yukinari Kato, Tetsuya Yamamoto, Akira Matsumura

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The molecular subgrouping of diffuse gliomas was recently found to stratify patients into prognostically distinct groups better than histological classification. Among several molecular parameters, the key molecules for the subtype diagnosis of diffuse gliomas are IDH mutation, 1p/19q co-deletion, and ATRX mutation; 1p/19q co-deletion is undetectable by immunohistochemistry, but is mutually exclusive with ATRX and p53 mutation in IDH mutant gliomas. Therefore, we applied ATRX and p53 immunohistochemistry instead of 1p/19q co-deletion analysis. The prognostic value of immunohistochemical diagnosis for Grade III gliomas was subsequently investigated. Then, the same immunohistochmical diagnostic approach was expanded for the evaluation of Grade II and IV diffuse glioma prognosis. The results indicate immunohistochemical analysis including IDH1/2, ATRX, p53, and Ki-67 index is valuable for the classification of diffuse gliomas, which is useful for the evaluation of prognosis, especially Grade III gliomas and lower-grade gliomas (i.e., Grade II and III).

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalBrain Tumor Pathology
Volume33
Issue number2
DOIs
Publication statusPublished - 2016 Apr 1
Externally publishedYes

Keywords

  • ATRX
  • Glioma
  • IDH
  • Immunohistochemistry
  • Ki-67 index
  • p53

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

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