TY - JOUR
T1 - Immunohistochemistry and in situ hybridization of P-450(17α) (17α- hydroxylase/17,20-lyase)
AU - Suzuki, T.
AU - Sasano, H.
AU - Sawai, T.
AU - Mason, J. I.
AU - Nagura, H.
PY - 1992
Y1 - 1992
N2 - Cytochrome P-450(17α) catalyzes both (17α)-hydroxylation and 17,20-side- chain cleavage in steroidogenesis and lies at a key branch point in the pathways of steroid hormone biosynthesis. To obtain information on the precise localization of P-450(17α) in swine testis, ovary, and adrenal, we undertook the simultaneous detection of P-450(17α) mRNA and protein by combining immunohistochemistry with in situ hybridization. In situ hybridization was performed on 4% paraformaldehyde-fixed, paraffin-embedded sections by employing either a 39-base oligomer or a cDNA insert (1.7 KB) of porcine testis P-450(17α) as DNA probe. Immunohistochemical study was performed by employing anti-P-450(17α). Hybridization signals were obtained in Leydig cells of the testis, theca interna of the ovarian follicle, and zona fasciculata reticularis cells of the adrenal cortex. Oligonucleotide probing yielded lower background signal than the cDNA probe. No specific signals were obtained in seminiferous tubules of the testis, medulla, and zona glomerulosa of the adrenal, and in membrana granulosa and interstitial cells of the ovary. Hybridization signals were obtained in the cells where immunoreactivity of the enzyme was observed by immunohistochemistry, except for some Leydig cells of the testis and theca interna cells of the ovary in which only immunoreactivity but not hybridization signal was observed. The present study provided detailed information about the precise cellular localization of P-450(17α) expression at both the protein and mRNA levels in swine adrenal glands and gonads. This approach of simultaneous immunohistochemistry and in situ hybridization analysis of steroidogenic enzymes can be applied in the future to tissues exhibiting abnormal steroid metabolism and should contribute to a better understanding of steroidogenesis.
AB - Cytochrome P-450(17α) catalyzes both (17α)-hydroxylation and 17,20-side- chain cleavage in steroidogenesis and lies at a key branch point in the pathways of steroid hormone biosynthesis. To obtain information on the precise localization of P-450(17α) in swine testis, ovary, and adrenal, we undertook the simultaneous detection of P-450(17α) mRNA and protein by combining immunohistochemistry with in situ hybridization. In situ hybridization was performed on 4% paraformaldehyde-fixed, paraffin-embedded sections by employing either a 39-base oligomer or a cDNA insert (1.7 KB) of porcine testis P-450(17α) as DNA probe. Immunohistochemical study was performed by employing anti-P-450(17α). Hybridization signals were obtained in Leydig cells of the testis, theca interna of the ovarian follicle, and zona fasciculata reticularis cells of the adrenal cortex. Oligonucleotide probing yielded lower background signal than the cDNA probe. No specific signals were obtained in seminiferous tubules of the testis, medulla, and zona glomerulosa of the adrenal, and in membrana granulosa and interstitial cells of the ovary. Hybridization signals were obtained in the cells where immunoreactivity of the enzyme was observed by immunohistochemistry, except for some Leydig cells of the testis and theca interna cells of the ovary in which only immunoreactivity but not hybridization signal was observed. The present study provided detailed information about the precise cellular localization of P-450(17α) expression at both the protein and mRNA levels in swine adrenal glands and gonads. This approach of simultaneous immunohistochemistry and in situ hybridization analysis of steroidogenic enzymes can be applied in the future to tissues exhibiting abnormal steroid metabolism and should contribute to a better understanding of steroidogenesis.
KW - Adrenal cortex
KW - Cytochrome P-450(17α)
KW - Immunohistochemistry
KW - In situ hybridization
KW - Ovary
KW - Swine
KW - Testis
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U2 - 10.1177/40.7.1607640
DO - 10.1177/40.7.1607640
M3 - Article
C2 - 1607640
AN - SCOPUS:0026764579
VL - 40
SP - 903
EP - 908
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 7
ER -