Immunohistochemical study of cytochrome P-45011 β-hydroxylase in human adrenal cortex with mineralo- and glucocorticoid excess

Hironobu Sasano, Mitsuhiro Okamoto, Nobuaki Sasano

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Cytochrome P-450 specific for steroid 11 β-hydroxylation (P-45011 β) was immunohistochemically demonstrated in the adrenal glands of human, pig and bovine and of mineralo- and glucocorticoid excess using a specific monoclonal antibody against P-45011 β of bovine adrenocortical mitochondria. P-45011 β was present in all three cortical zones of the histologically normal adrenal glands of bovine, pig and human, particularly in the zona fasciculata (ZF) and reticularis (ZR). The P-45011 β immunoreactivity was intensive in cortical micronodules and inner ZF and ZR in Cushing's disease, and relatively intensive in the zona glomerulosa (ZG) and outer ZF in idiopathic hyperaldosteronism (IHA), corresponding to the sites of active steroidogenesis. In adenomas with Cushing's syndrome and primary aldosteronism, compact cells were generally stained well. In the adrenal glands attached to the adenomas, immunoreactivity was observed only focally in ZG cells but not in ZF and ZR cells.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Issue number4
Publication statusPublished - 1988 Jul 1


  • Adrenal cortex
  • Adrenocortical adenoma
  • Cytochrome P-450
  • Hypercorticism
  • Steroid 11 β-hydroxylase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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