Immunohistochemical study of cytochrome P-45011 β-hydroxylase in human adrenal cortex with mineralo- and glucocorticoid excess

Hironobu Sasano, Mitsuhiro Okamoto, Nobuaki Sasano

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cytochrome P-450 specific for steroid 11 β-hydroxylation (P-45011 β) was immunohistochemically demonstrated in the adrenal glands of human, pig and bovine and of mineralo- and glucocorticoid excess using a specific monoclonal antibody against P-45011 β of bovine adrenocortical mitochondria. P-45011 β was present in all three cortical zones of the histologically normal adrenal glands of bovine, pig and human, particularly in the zona fasciculata (ZF) and reticularis (ZR). The P-45011 β immunoreactivity was intensive in cortical micronodules and inner ZF and ZR in Cushing's disease, and relatively intensive in the zona glomerulosa (ZG) and outer ZF in idiopathic hyperaldosteronism (IHA), corresponding to the sites of active steroidogenesis. In adenomas with Cushing's syndrome and primary aldosteronism, compact cells were generally stained well. In the adrenal glands attached to the adenomas, immunoreactivity was observed only focally in ZG cells but not in ZF and ZR cells.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Volume413
Issue number4
DOIs
Publication statusPublished - 1988 Jul 1

Keywords

  • Adrenal cortex
  • Adrenocortical adenoma
  • Cytochrome P-450
  • Hypercorticism
  • Steroid 11 β-hydroxylase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Immunohistochemical study of cytochrome P-450<sub>11 β</sub>-hydroxylase in human adrenal cortex with mineralo- and glucocorticoid excess'. Together they form a unique fingerprint.

  • Cite this