TY - JOUR
T1 - Immunohistochemical studies on EGF family growth factors in normal and ulcerated human gastric mucosa
AU - Abe, Shinya
AU - Sasano, Hironobu
AU - Katoh, Katsuaki
AU - Ohara, Shuichi
AU - Arikawa, Tadashi
AU - Noguchi, Tetsuya
AU - Asaki, Shigeru
AU - Yasui, Wataru
AU - Tahara, Eiichi
AU - Nagura, Hiroshi
AU - Toyota, Takayoshi
PY - 1997
Y1 - 1997
N2 - Expression of members of the epidermal growth factor family, including epidermal growth factor (EGF), transforming growth factor-α (TGF-α), amphiregulin (AR), and Cripto, as well as their putative receptor, epidermal growth factor receptor (EGFR), was studied immunohistochemically in human gastric mucosa to evaluate their possible roles in cell proliferation of normal and regenerative gastric mucosa. We also examined the correlation between cell proliferation and EGFR by double immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and EGFR. In normal gastric mucosa, TGF-α, Cripto, and AR immunoreactivities were observed in the surface epithelial and parietal cells of gastric fundic glands, respectively. EGF immunoreactivity was not observed in any of normal mucosa examined. EGFR immunoreactivity was detected on foveolar cells in proliferative zones and in parietal cells. Double immunostaining revealed that EGFR immunoreactivity was distributed much more widely than PCNA immunoreactivity. PCNA positive epithelial cells adjacent to gastric ulcer margin expressed relatively intense EGFR but did not express any of the growth factors examined. On the other hand, relatively intense immunoreactivity of both TGF-α and Cripto was detected in PCNA-negative regenerative epithelium located distant from gastric ulcer margin. Relative immunoreactivity of AR in regenerative gastric epithelium associated with ulcer was not different from that in normal gastric mucosa. TGF-α, AR, and Cripto are considered to play important roles in normal gastric mucosal proliferation, and TGF-α and Cripto may be involved in ulcer healing, possibly via a paracrine mechanism.
AB - Expression of members of the epidermal growth factor family, including epidermal growth factor (EGF), transforming growth factor-α (TGF-α), amphiregulin (AR), and Cripto, as well as their putative receptor, epidermal growth factor receptor (EGFR), was studied immunohistochemically in human gastric mucosa to evaluate their possible roles in cell proliferation of normal and regenerative gastric mucosa. We also examined the correlation between cell proliferation and EGFR by double immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and EGFR. In normal gastric mucosa, TGF-α, Cripto, and AR immunoreactivities were observed in the surface epithelial and parietal cells of gastric fundic glands, respectively. EGF immunoreactivity was not observed in any of normal mucosa examined. EGFR immunoreactivity was detected on foveolar cells in proliferative zones and in parietal cells. Double immunostaining revealed that EGFR immunoreactivity was distributed much more widely than PCNA immunoreactivity. PCNA positive epithelial cells adjacent to gastric ulcer margin expressed relatively intense EGFR but did not express any of the growth factors examined. On the other hand, relatively intense immunoreactivity of both TGF-α and Cripto was detected in PCNA-negative regenerative epithelium located distant from gastric ulcer margin. Relative immunoreactivity of AR in regenerative gastric epithelium associated with ulcer was not different from that in normal gastric mucosa. TGF-α, AR, and Cripto are considered to play important roles in normal gastric mucosal proliferation, and TGF-α and Cripto may be involved in ulcer healing, possibly via a paracrine mechanism.
KW - Amphiregulin
KW - Cripto
KW - Epidermal growth factor
KW - Epidermal growth factor receptor
KW - Gastric ulcer
KW - Transforming growth factor- α
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U2 - 10.1023/A:1018897922644
DO - 10.1023/A:1018897922644
M3 - Article
C2 - 9201085
AN - SCOPUS:8544229079
VL - 42
SP - 1199
EP - 1209
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 6
ER -