Immunohistochemical localization of thromboxane receptor and thromboxane synthase in rat testis

Nobuyuki Takahashi, Kazuhisa Takeuchi, Takaaki Abe, Kazuhiro Murakami, Masaaki Yamaguchi, Keishi Abe

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The cellular localization of thromboxane A2 receptor (TXR) and thromboxane synthase (TXS) in rat testes was examined with an antibody against the carboxyl-terminal tail of rat TXR and anti-porcine lung TXS antibody. By light microscopy immunoreactivity for TXR was shown to be present in spermatids, whereas spermatogonia, spermatocytes, and spermatozoa lacked the immunoreactivity. Immunoelectron microscopic analysis revealed that immunostainable TXR was present in acrosomes of spermatids. In contrast, immunoreactivity for TXS was present in all stages of spermatogenic cells; spermatogonia, spermatocytes, spermatids, and spermatozoa. TX system may possibly contribute to the formation of acrosomes or have some unrecognized functions in an autocrine/paracrine fashion.

Original languageEnglish
Pages (from-to)4143-4146
Number of pages4
JournalEndocrinology
Volume136
Issue number9
DOIs
Publication statusPublished - 1995 Sep

ASJC Scopus subject areas

  • Endocrinology

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