TY - JOUR
T1 - Immunohistochemical localization of thromboxane receptor and thromboxane synthase in rat testis
AU - Takahashi, Nobuyuki
AU - Takeuchi, Kazuhisa
AU - Abe, Takaaki
AU - Murakami, Kazuhiro
AU - Yamaguchi, Masaaki
AU - Abe, Keishi
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1995/9
Y1 - 1995/9
N2 - The cellular localization of thromboxane A2 receptor (TXR) and thromboxane synthase (TXS) in rat testes was examined with an antibody against the carboxyl-terminal tail of rat TXR and anti-porcine lung TXS antibody. By light microscopy immunoreactivity for TXR was shown to be present in spermatids, whereas spermatogonia, spermatocytes, and spermatozoa lacked the immunoreactivity. Immunoelectron microscopic analysis revealed that immunostainable TXR was present in acrosomes of spermatids. In contrast, immunoreactivity for TXS was present in all stages of spermatogenic cells; spermatogonia, spermatocytes, spermatids, and spermatozoa. TX system may possibly contribute to the formation of acrosomes or have some unrecognized functions in an autocrine/paracrine fashion.
AB - The cellular localization of thromboxane A2 receptor (TXR) and thromboxane synthase (TXS) in rat testes was examined with an antibody against the carboxyl-terminal tail of rat TXR and anti-porcine lung TXS antibody. By light microscopy immunoreactivity for TXR was shown to be present in spermatids, whereas spermatogonia, spermatocytes, and spermatozoa lacked the immunoreactivity. Immunoelectron microscopic analysis revealed that immunostainable TXR was present in acrosomes of spermatids. In contrast, immunoreactivity for TXS was present in all stages of spermatogenic cells; spermatogonia, spermatocytes, spermatids, and spermatozoa. TX system may possibly contribute to the formation of acrosomes or have some unrecognized functions in an autocrine/paracrine fashion.
UR - http://www.scopus.com/inward/record.url?scp=0029080758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029080758&partnerID=8YFLogxK
U2 - 10.1210/endo.136.9.7649122
DO - 10.1210/endo.136.9.7649122
M3 - Article
C2 - 7649122
AN - SCOPUS:0029080758
SN - 0013-7227
VL - 136
SP - 4143
EP - 4146
JO - Endocrinology
JF - Endocrinology
IS - 9
ER -