TY - JOUR
T1 - Immunohistochemical Localization of Mineralocorticoid Receptor in Human Gut
AU - Fukushima, Kouhei
AU - Sasaki, Iwao
AU - Matsuno, Seiki
AU - Sasano, Hironobu
AU - Nagura, Hiroshi
AU - Krozowski, Zygmund S.
PY - 1991
Y1 - 1991
N2 - Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100 kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.
AB - Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100 kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.
KW - human gut
KW - immunohistochemistry
KW - mineralocorticoid receptor
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U2 - 10.1620/tjem.165.155
DO - 10.1620/tjem.165.155
M3 - Article
C2 - 1667443
AN - SCOPUS:0026234694
VL - 165
SP - 155
EP - 163
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
SN - 0040-8727
IS - 2
ER -