Immunohistochemical Localization of Mineralocorticoid Receptor in Human Gut

Kouhei Fukushima; Iwao Sasaki; Seiki Matsuno; Hironobu Sasano; Hiroshi Nagura; Zygmund S. Krozowski

doi:10.1620/tjem.165.155

Immunohistochemical Localization of Mineralocorticoid Receptor in Human Gut

Kouhei Fukushima, Iwao Sasaki, Seiki Matsuno, Hironobu Sasano, Hiroshi Nagura, Zygmund S. Krozowski

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100 kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.

Original language	English
Pages (from-to)	155-163
Number of pages	9
Journal	Tohoku Journal of Experimental Medicine
Volume	165
Issue number	2
DOIs	https://doi.org/10.1620/tjem.165.155
Publication status	Published - 1991

Keywords

human gut
immunohistochemistry
mineralocorticoid receptor

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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abstract = "Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100 kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.",

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AU - Fukushima, Kouhei

AU - Sasaki, Iwao

AU - Matsuno, Seiki

AU - Sasano, Hironobu

AU - Nagura, Hiroshi

AU - Krozowski, Zygmund S.

PY - 1991

Y1 - 1991

N2 - Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100 kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.

AB - Immunohistochemical localization of mineralocorticoid receptor in the human adult gut was examined by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein. Immunoreactive staining was observed in columnar cells in the middle of the intestinal glands of the proximal colon, but not in goblet cells. Western blot analysis also revealed the presence of an approximately 100 kDa immunoreactive species consistent with the mineralocorticoid receptor protein in the ascending colon as well as the kidney. These results suggest that aldosterone-sensitive sodium transport and water absorption may be mainly carried out in the proximal colon in human adult gut at non-pathological state.

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KW - immunohistochemistry

KW - mineralocorticoid receptor

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Immunohistochemical Localization of Mineralocorticoid Receptor in Human Gut

Abstract

Keywords

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