Immunohistochemical localization of cytochrome P-450C21 in human adrenal cortex and its relation to endocrine function

Hironobu Sasano, Perrin C. White, Maria I. New, Nobuaki Sasano

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41 Citations (Scopus)


Cytochrome P-450C21 was successfully demonstrated in the human adrenal glands by a peroxidase-antiperoxidase method. All three cortical layers were stained in the normal adrenal glands, particularly the zonae glomerulosa and reticularis. Well-stained and faintly stained cells were intermingled in the zona fasciculata, suggestive of intrazonal variations. The immunoreactivity was particularly intense at the site of ACTH action, i.e., cells in micronodules and cells around myelolipomatous lesions in adrenocortical hyperplasia of Cushing's disease and sites of regeneration in the normal adrenal glands. In adrenocortical adenomas with Cushing's syndrome and primary aldosteronism, cells with large nuclei were generally stained well. In the adrenocortical tissue adjacent to a functioning adenoma, the immunore-activity was observed only in the zona glomerulosa, especially in cases of primary aldosteronism. This finding is consistent with morphologic observations.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalHuman Pathology
Issue number2
Publication statusPublished - 1988 Feb
Externally publishedYes


  • adrenal gland
  • adrenal tumors
  • cytochrome P-450
  • steroidogenesis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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