TY - JOUR
T1 - Immunohistochemical distribution of 11β-hydroxysteroid dehydrogenase in human eye
AU - Suzuki, Takashi
AU - Sasano, Hironobu
AU - Kaneko, Chika
AU - Ogawa, Sayaka
AU - Darnel, Andrew D.
AU - Krozowski, Zygmunt S.
PY - 2001/2/22
Y1 - 2001/2/22
N2 - 11β-hydroxysteroid dehydrogenase (11β-HSD) regulates local actions of corticosteroids at glucocorticoid and mineralocorticoid receptors. Corticosteroids are thought to play important roles in ocular function. However, mechanisms of intraocular corticosteroid action are still unclear. Therefore, in this study, we examined the immunohistochemical localization of 11β-HSD type 1 (11β-HSD1), 11β-HSD type 2 (11β-HSD2), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in human ocular tissues from patients (6 months to 78 years of age; n=10) retrieved from surgical pathology files. Both 11β-HSD2 and MR immunoreactivity was detected only in non-pigmented epithelium of the ciliary body, but was undetectable in cornea, lens, iris, retina, choroid and sclera, in all the cases examined. GR was detected in all cell types in the human eye. 11β-HSD1 immunoreactivity was not detected in the human eye in this study. These results suggest that 11β-HSD2 play an important role in human ocular mineralocorticoid action, such as the production of aqueous humor, in the ciliary body. The widespread expression of GR suggests that glucocorticoids may play an important role in the function and homeostasis of the human eye.
AB - 11β-hydroxysteroid dehydrogenase (11β-HSD) regulates local actions of corticosteroids at glucocorticoid and mineralocorticoid receptors. Corticosteroids are thought to play important roles in ocular function. However, mechanisms of intraocular corticosteroid action are still unclear. Therefore, in this study, we examined the immunohistochemical localization of 11β-HSD type 1 (11β-HSD1), 11β-HSD type 2 (11β-HSD2), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in human ocular tissues from patients (6 months to 78 years of age; n=10) retrieved from surgical pathology files. Both 11β-HSD2 and MR immunoreactivity was detected only in non-pigmented epithelium of the ciliary body, but was undetectable in cornea, lens, iris, retina, choroid and sclera, in all the cases examined. GR was detected in all cell types in the human eye. 11β-HSD1 immunoreactivity was not detected in the human eye in this study. These results suggest that 11β-HSD2 play an important role in human ocular mineralocorticoid action, such as the production of aqueous humor, in the ciliary body. The widespread expression of GR suggests that glucocorticoids may play an important role in the function and homeostasis of the human eye.
KW - 11β-HSD
KW - Human eye
KW - Immunohistochemistry
KW - Mineralocorticoid
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U2 - 10.1016/S0303-7207(00)00403-2
DO - 10.1016/S0303-7207(00)00403-2
M3 - Article
C2 - 11223183
AN - SCOPUS:0035931777
VL - 173
SP - 121
EP - 125
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -