Immunohistochemical distribution and functional characterization of an organic anion transporting polypeptide 2 (oatp2)

Masayuki Kakyo, Hiroyuki Sakagami, Toshiyuki Nishio, Daisuke Nakai, Rie Nakagomi, Taro Tokui, Takeshi Naitoh, Seiki Matsuno, Takaaki Abe, Hiromu Yawo

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

The rabbit polyclonal antibody against rat organic anion transporting polypeptide 2 (oatp2) was raised and immunoaffinity-purified. Western blot analysis for oatp2 detected two bands (~74 and 76 kDa) in rat brain and a single band (76 kDa) in the liver. By immunohistochemical analysis, the oatp2 immunoreactivity was specifically high at the basolateral membrane of rat hepatocytes. Functionally, the oatp2-expressing oocytes were found to transport dehydroepiandrosterone sulfate, δ1 opioid receptor agonist [d-Pen2,d-Pen5]enkephalin, Leu-enkephalin, and biotin significantly, as well as the substrates previously reported. These data reveal the exact distribution of the rat oatp2 at the protein level in the liver, and that oatp2 appears to be involved in the multispecificity of the uptaking substrates in the liver and brain. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalFEBS Letters
Volume445
Issue number2-3
DOIs
Publication statusPublished - 1999 Feb 26

Keywords

  • Basolateral membrane
  • Dehydroepiandrosterone sulfate
  • Organic anion transporting polypeptide 2
  • Rat liver
  • Steroid hormone
  • Thyroid hormone
  • [d-Pen,d-Pen]Enkephalin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Immunohistochemical distribution and functional characterization of an organic anion transporting polypeptide 2 (oatp2)'. Together they form a unique fingerprint.

Cite this