Immunohistochemical detection of IDH1 mutation, p53, and internexin as prognostic factors of glial tumors

Shingo Takano, Yukinari Kato, Tetsuya Yamamoto, Mika Kato Kaneko, Eiichi Ishikawa, Yuta Tsujimoto, Masahide Matsuda, Kei Nakai, Ryo Yanagiya, Shunpei Morita, Koji Tsuboi, Akira Matsumura

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Isocitrate dehydrogenase 1 (IDH1) mutations, which are early and frequent genetic alterations in astrocytomas, oligodendrogliomas, oligoastrocytomas, and secondary glioblastomas, are specific to arginine 132 (R132). Recently, we established monoclonal antibodies (mAbs) against IDH1 mutations: anti-IDH1-R132H and anti-IDH1-R132S. However, the importance of immunohistochemistry using the combination of those mAbs has not been elucidated. For this study, 164 cases of glioma were evaluated immunohistochemically for IDH1 mutations (R132H and R132S) using anti-IDH1 mAbs (HMab-1 and SMab-1). IDH1 mutation was detected, respectively, in 9.7%, 63.6%, 51.7%, and 77.8% of primary grade IV, secondary grade IV, grade III, and grade II gliomas. For each grade of glioma, prognostic factors for progressionfree survival and overall survival were evaluated using clinical and pathological parameters in addition to IDH1 immunohistochemistry. IDH1 mutation, p53 overexpression, and internexin expression, as evaluated using immunohistochemistry with clinical parameters such as degree of surgical removal and preoperative Karnofsky Performance Status (KPS), might be of greater prognostic significance than histological grading alone in grade III as well as IDH1 mutation in grade IV gliomas.

Original languageEnglish
Pages (from-to)361-373
Number of pages13
JournalJournal of Neuro-Oncology
Volume108
Issue number3
DOIs
Publication statusPublished - 2012 Jul
Externally publishedYes

Keywords

  • Glioma
  • IDH1
  • Immunohistochemistry
  • Internexin
  • Monoclonal antibody
  • Mutation
  • P53

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Immunohistochemical detection of IDH1 mutation, p53, and internexin as prognostic factors of glial tumors'. Together they form a unique fingerprint.

Cite this