Immunohistochemical detection of advanced glycosylation end-products in the peritoneum and its possible pathophysiological role in CAPD

Masaaki Nakayama, Yoshindo Kawaguchi, Kenichi Yamada, Toshio Hasegawa, Kazunori Takazoe, Naohiko Katoh, Hiroshi Hayakawa, Naoyuki Osaka, Hiroyasu Yamamoto, Aiichiro Ogawa, Hitoshi Kubo, Takashi Shigematsu, Osamu Sakai, Seikou Horiuchi

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274 Citations (Scopus)

Abstract

It has recently been suggested that advanced glycosylation end-products (AGEs) are formed in the peritoneum in patients on CAPD. However, the exact location of AGE accumulation, the relation with the duration of CAPD and its pathophysiological role in CAPD remain unclear. If the peritoneum is glycosylated, it could bring about altered peritoneal function. Therefore, the aim of this study is to clarify the localization of AGEs in the peritoneum in accordance with the duration of CAPD and to examine its relation to the peritoneal permeability. Fifteen non-diabetic patients were divided into three groups (each 5 patients) on the basis of the mean duration (D) of CAPD (Group I, D = 0 month; Group II, D = 34 months; Group III, D = 84 months). The AGE staining by monoclonal anti-AGE antibody in the peritoneum and the four-hour peritoneal equilibration test (PET) were compared among these groups. AGE was absent or found only weakly in Group I. However, in groups II and III, AGE was moderately or strongly positive especially in the vascular walls and it was dominant in Group III. PET revealed that peritoneal permeability for glucose, creatinine, β2-microglobulin and albumin was increased in Group II as compared to Group I, and it was further increased in Group III. The results of this study indicate that AGEs become dominantly accumulated in the vascular wall in accordance with the prolongation of CAPD treatment, and this might play some roles for the increased permeability of the peritoneal membrane in CAPD.

Original languageEnglish
Pages (from-to)182-186
Number of pages5
JournalKidney international
Volume51
Issue number1
DOIs
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Nephrology

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