Immunohistochemical Demonstration of Cholesterol Side-chain Cleavage Cytochrome P-450 in Bovine and Human Adrenals

Hironobu Sasano, Nobuaki Sasano, Mitsuhiro Okamoto

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cytochrome P-450 specific for cholesterol side-chain cleavage (P-450SCC) was purified from the bovine adrenal and a specific antibody was raised in rabbits. The antiserum was applied for immunohistochemical visualization of the P-450SCC in the bovine and human adrenal cortex. The immunoreactivity was intense in the zona fasciculata (ZF) and reticularis (ZR) while weak in the zona glomerulosa (ZG) in the normal adrenals. In adrenocortical hyperplasia, a marked immunoreactivity was observed in the ZG in idiopathic hyperaldosteronism and the inner ZF and reticularis particularly in cells of micronodules in Cushing's disease, corresponding to cells with active steroidogenesis. In aldosteronoma and adenoma with Cushing's syndrome, P-450SCC was generally present in compact cells of adenomas.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalPathology Research and Practice
Volume184
Issue number3
DOIs
Publication statusPublished - 1989 Jan 1

Keywords

  • Adrenocortical disorders
  • Cytochrome P-450-Cholesterol side-chain cleavag
  • Steroidogenesis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

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