Immunohistochemical analysis of cytochrome P-450 17α-hydroxylase in pig adrenal cortex, testis and ovary

Sasano Hironobu, J. Ian Mason, Sasano Nobuaki

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32 Citations (Scopus)

Abstract

Cytochrome P-450 specific for steroid 17α-hydroxylation (P-45017α) was immunohistochemically observed in pig adrenal cortex, testis and ovary by the biotin-streptavidin method using a specific antibody against P-45017α purified from neonatal pig testis. In the adrenal cortex, P-45017α was present in the zona fasciculata and reticularis while no immunoreactivity was observed in the zona glomerulosa, confirming the absence of 17-hydroxylated steroid synthesis in the zona glomerulosa. In the testis, P-45017α was present exclusively in Leydig cells and immunoreactivity was absent in seminiferous tubules. In the ovary, immunoreactivity was observed only in the theca interna but not in the membrana granulosa of follicles. Among the tissues examined, the relative intensity of immunoreactivity was greatest in the Leydig cells, and progressively less in theca interna cells, outer fasciculata cells and inner fasciculata and reticularis cells.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume62
Issue number2
DOIs
Publication statusPublished - 1989 Apr

Keywords

  • (Pig ovary, testis and adrenal cortex)
  • 17α-Hydroxylase
  • Cytochrome P-450
  • Immunohistochemistry

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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