Immunohistochemical analyses of tumor-associated glycoprotein in gastric and breast carcinomas

N. Ohuchi, H. Ohtani, J. Schlom, S. Mori

Research output: Contribution to journalArticle

Abstract

The distribution and localization of tumor-associated glycoprotein, TAG-72, in gastric and breast carcinomas were described utilizing monoclonal antibody (MoAb) B72.3 and immunohistochemical analyses. The light immunohistochemistry demonstrated the preferential expression of TAG-72 in carcinoma versus benign or normal epithelial cells of the breast. The TAG-72 was expressed in adenocarcinomas as well as dysplastic lesions or epithelium with intestinal metaplasia of the stomach. The immunoelectron microscopy in breast tissues demonstrated the ultrastructural localization of TAG-72 on cell membrane, secretory granules, rER or Golgi complex in carcinoma cells, but no localization in benign epithelial cells. The immunoelectron microscopy in gastric tissues revealed the localization of TAG-72 on cell membrane, secretory granules, rER, or Golgi complex in carcinoma cells, and occasionally on rER, or Golgi complex in foveolar epithelial cells. The TAG-72 may be synthesized in rER and Golgi complex and the localization along the cell membrane, or in secretory granules may be specific for carcinoma cells.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalJournal of Tumor Marker Oncology
Volume4
Issue number4
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Cancer Research

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