Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney

Kazuhiro Takahashi; Kumi Kikuchi; Yutaka Maruyama; Tomoko Urabe; Kiichiro Nakajima; Hironobu Sasano; Yutaka Imai; Osamu Murakami; Kazuhito Totsune

doi:10.1016/j.peptides.2005.11.004

Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney

Kazuhiro Takahashi, Kumi Kikuchi, Yutaka Maruyama, Tomoko Urabe, Kiichiro Nakajima, Hironobu Sasano, Yutaka Imai, Osamu Murakami, Kazuhito Totsune

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.

Original language	English
Pages (from-to)	1383-1389
Number of pages	7
Journal	Peptides
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.peptides.2005.11.004
Publication status	Published - 2006 Jun

Keywords

Adrenomedullin
Heart
Hypothalamus
Immunocytochemistry
Intermedin
Kidney

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney. / Takahashi, Kazuhiro; Kikuchi, Kumi; Maruyama, Yutaka; Urabe, Tomoko; Nakajima, Kiichiro; Sasano, Hironobu; Imai, Yutaka; Murakami, Osamu; Totsune, Kazuhito.

In: Peptides, Vol. 27, No. 6, 06.2006, p. 1383-1389.

Research output: Contribution to journal › Article › peer-review

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title = "Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney",

abstract = "Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.",

keywords = "Adrenomedullin, Heart, Hypothalamus, Immunocytochemistry, Intermedin, Kidney",

author = "Kazuhiro Takahashi and Kumi Kikuchi and Yutaka Maruyama and Tomoko Urabe and Kiichiro Nakajima and Hironobu Sasano and Yutaka Imai and Osamu Murakami and Kazuhito Totsune",

note = "Funding Information: The authors are grateful to Dr. T. Mouri (Mouri Clinic, Natori Japan) for his gift of the antiserum against arginine vasopressin and Dr. T.X. Watanabe (Peptide Institute, Minoh-Shi, Japan) for his helpful comments on the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture of Japan, a Research Grant from the HIROMI Medical Research Foundation (2001), a Research Grant from the Intelligent Cosmos (2002, 2003) and a Research Grant from the Takeda Research Foundation (2005).",

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T1 - Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney

AU - Takahashi, Kazuhiro

AU - Kikuchi, Kumi

AU - Maruyama, Yutaka

AU - Urabe, Tomoko

AU - Nakajima, Kiichiro

AU - Sasano, Hironobu

AU - Imai, Yutaka

AU - Murakami, Osamu

AU - Totsune, Kazuhito

N1 - Funding Information: The authors are grateful to Dr. T. Mouri (Mouri Clinic, Natori Japan) for his gift of the antiserum against arginine vasopressin and Dr. T.X. Watanabe (Peptide Institute, Minoh-Shi, Japan) for his helpful comments on the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture of Japan, a Research Grant from the HIROMI Medical Research Foundation (2001), a Research Grant from the Intelligent Cosmos (2002, 2003) and a Research Grant from the Takeda Research Foundation (2005).

PY - 2006/6

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N2 - Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.

AB - Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.

KW - Adrenomedullin

KW - Heart

KW - Hypothalamus

KW - Immunocytochemistry

KW - Intermedin

KW - Kidney

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