Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney

Kazuhiro Takahashi, Kumi Kikuchi, Yutaka Maruyama, Tomoko Urabe, Kiichiro Nakajima, Hironobu Sasano, Yutaka Imai, Osamu Murakami, Kazuhito Totsune

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.

Original languageEnglish
Pages (from-to)1383-1389
Number of pages7
JournalPeptides
Volume27
Issue number6
DOIs
Publication statusPublished - 2006 Jun

Keywords

  • Adrenomedullin
  • Heart
  • Hypothalamus
  • Immunocytochemistry
  • Intermedin
  • Kidney

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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