TY - JOUR
T1 - Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
AU - Hosoya, Shoichi
AU - Villena, Julio
AU - Shimazu, Tomoyuki
AU - Tohno, Masanori
AU - Fujie, Hitomi
AU - Chiba, Eriko
AU - Shimosato, Takeshi
AU - Aso, Hisashi
AU - Suda, Yoshihito
AU - Kawai, Yasushi
AU - Saito, Tadao
AU - Alvarez, Susana
AU - Ikegami, Shuji
AU - Itoh, Hiroyuki
AU - Kitazawa, Haruki
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (B)(2) (No. 21380164) from the Japan Society for the Promotion of Science (JSPS), the Kieikai Research Foundation and Japan Racing Association to Dr H. Kitazawa. Dr Julio Villena was supported by JSPS (Postdoctoral Fellowship for Foreign Researchers, Program No. 21-09335).
PY - 2011
Y1 - 2011
N2 - This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
AB - This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
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U2 - 10.1186/1297-9716-42-111
DO - 10.1186/1297-9716-42-111
M3 - Article
C2 - 22046952
AN - SCOPUS:84858123087
SN - 0928-4249
VL - 42
JO - Annales de Recherches Veterinaires
JF - Annales de Recherches Veterinaires
IS - 1
M1 - 111
ER -