Immunobiological activities of a chemically synthesized lipid A of Porphyromonas gingivalis

Tomohiko Ogawa, Yasuyuki Asai, Hiroyo Yamamoto, Yasuhiro Taiji, Takayoshi Jinno, Tohru Kodama, Shinjiro Niwata, Hidetoshi Shimauchi, Kuniyasu Ochiai

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A synthetic lipid A of Porphyromonas gingivalis strain 381 (compound PG-381), which is similar to its natural lipid A, demonstrated no or very low endotoxic activities as compared to Escherichia coli-type synthetic lipid A (compound 506). On the other hand, compound PG-381 had stronger hemagglutinating activities on rabbit erythrocytes than compound 506. Compound PG-381 also induced mitogenic responses in spleen cells from lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice, as well as LPS-responsive C3H/HeN mice. The addition of polymyxin B resulted in the inhibition of mitogenic activities, however, compound 506 did not show these capacities. Additionally, compound PG-381 showed a lower level of activity in inducing cytokine production in peritoneal macrophages and gingival fibroblasts from C3H/HeN mice, but not C3H/HeJ mice, in comparison to compound 506. Thus, this study demonstrates that the chemical synthesis of lipid A, mimicking the natural lipid A portion of LPS from P. gingivalis, confirms its low endotoxic potency and immunobiological activity. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume28
Issue number4
DOIs
Publication statusPublished - 2000 Aug

Keywords

  • C3H/HeJ mouse
  • Endotoxicity
  • Gingival fibroblast
  • Macrophage
  • Porphyromonas gingivalis
  • Synthetic lipid A

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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