Immunoassay of the MRSA-related toxic protein, leukocidin, with scanning electrochemical microscopy

S. Kasai, A. Yokota, H. Zhou, M. Nishizawa, K. Niwa, T. Onouchi, T. Matsue

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Scanning electrochemical microscopy (SECM) was applied to the immunoassay of leukocidin, which is a toxic protein produced by methicillin-resistant Staphylococcus aureus (MRSA), with the intention of developing and early diagnostic for MRSA infection. An antibody - chip for leukocidin was prepared by self-assembling of anti-leukocidin on a protein A-coated glass substrate. A sample solution containing leukocidin was spotted onto the antibody-chip, followed by labeling with horseradish peroxidase (HRP) via a sandwich method. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images of the spot of captured leukocidin. The amplitude of reduction current depended on the concentrations of sample solutions used for making spots. This SECM-based immunoassay detects as low as 5.25 pg mL-1 leukocidin.

Original languageEnglish
Pages (from-to)5761-5765
Number of pages5
JournalAnalytical Chemistry
Volume72
Issue number23
DOIs
Publication statusPublished - 2000 Dec 1

ASJC Scopus subject areas

  • Analytical Chemistry

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