Immuno-wall lab-on-a-chip protein analysis devices for high precision surgery of gliomas

T. Kasama, A. Yamamichi, F. Ohka, Y. Kato, H. Suzuki, A. Kato, K. Motomura, M. Hirano, M. Ranjit, L. Chalise, M. Kurimoto, G. Kondo, K. Aoki, N. Kaji, T. Matsubara, H. Suzuki, M. Tokeshi, T. Wakabayashi, A. Natsume, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In the present study, we propose immuno-wall lab-on-a-chip protein analysis devices for ultrarapid detection of R132H mutant isocitrate dehydrogenase 1 (IDH1) that we identified as a specifically and commonly expressed gene in diffusely infiltrative gliomas1. In 9 min immunoassay, we successfully carried out specific detection of R132H mutant IDH1 in a lysate of glioma tissue, which is much faster than intraoperative pathological diagnosis. Moreover, clinical samples from 10 patients with gliomas were analyzed by using the immuno-wall devices. These results were consistent with those of PCR-based genetic mutation detection method. Our devices realize point-of-care tumor testing, resulting in the high precision surgery of gliomas.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages651-652
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Glioma
  • Immunoassay
  • Isocitrate dehydrogenase 1
  • R132h mutation

ASJC Scopus subject areas

  • Control and Systems Engineering

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