Immortalization by large T-antigen of the adult epididymal duct epithelium

Christiane Kirchhoff, Yoshihiko Araki, Ilpo Huhtaniemi, Robert J. Matusik, Caroline Osterhoff, Matti Poutanen, Annemarie Samalecos, Petra Sipilä, Kichiya Suzuki, Marie Claire Orgebin-Crist

Research output: Contribution to journalReview articlepeer-review

21 Citations (Scopus)

Abstract

The SV40 large T-antigen has been widely used to convert various cell types to a transformed phenotype, and also to induce progressive tumours in transgenic animals. The objectives of this review are to compare and discuss three different approaches to generate epididymal epithelial cell lines using the large T-antigen. In the first approach, retroviral transfection of primary cultures was used to immortalize canine epididymal cells in vitro; the other two approaches used transgenic mice expressing the large T-antigen. In one of these in vivo approaches, a construct consisting of the coding sequence of a temperature sensitive (ts) SV40 large T-antigen was inserted in a mouse genome. When the cells are exposed to the permissive temperature of 33°C, functional expression of the large T-antigen occurs and cells start to proliferate. In the second in vivo approach a tissue-specific promoter, the 5kb GPX5 promoter, was used to direct expression of the large T-antigen to the epididymal duct epithelium.

Original languageEnglish
Pages (from-to)83-94
Number of pages12
JournalMolecular and Cellular Endocrinology
Volume216
Issue number1
DOIs
Publication statusPublished - 2004 Mar 15
Externally publishedYes

Keywords

  • Epididymal
  • Immortalization
  • T-antigen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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