TY - JOUR
T1 - Immortalization by large T-antigen of the adult epididymal duct epithelium
AU - Kirchhoff, Christiane
AU - Araki, Yoshihiko
AU - Huhtaniemi, Ilpo
AU - Matusik, Robert J.
AU - Osterhoff, Caroline
AU - Poutanen, Matti
AU - Samalecos, Annemarie
AU - Sipilä, Petra
AU - Suzuki, Kichiya
AU - Orgebin-Crist, Marie Claire
PY - 2004/3/15
Y1 - 2004/3/15
N2 - The SV40 large T-antigen has been widely used to convert various cell types to a transformed phenotype, and also to induce progressive tumours in transgenic animals. The objectives of this review are to compare and discuss three different approaches to generate epididymal epithelial cell lines using the large T-antigen. In the first approach, retroviral transfection of primary cultures was used to immortalize canine epididymal cells in vitro; the other two approaches used transgenic mice expressing the large T-antigen. In one of these in vivo approaches, a construct consisting of the coding sequence of a temperature sensitive (ts) SV40 large T-antigen was inserted in a mouse genome. When the cells are exposed to the permissive temperature of 33°C, functional expression of the large T-antigen occurs and cells start to proliferate. In the second in vivo approach a tissue-specific promoter, the 5kb GPX5 promoter, was used to direct expression of the large T-antigen to the epididymal duct epithelium.
AB - The SV40 large T-antigen has been widely used to convert various cell types to a transformed phenotype, and also to induce progressive tumours in transgenic animals. The objectives of this review are to compare and discuss three different approaches to generate epididymal epithelial cell lines using the large T-antigen. In the first approach, retroviral transfection of primary cultures was used to immortalize canine epididymal cells in vitro; the other two approaches used transgenic mice expressing the large T-antigen. In one of these in vivo approaches, a construct consisting of the coding sequence of a temperature sensitive (ts) SV40 large T-antigen was inserted in a mouse genome. When the cells are exposed to the permissive temperature of 33°C, functional expression of the large T-antigen occurs and cells start to proliferate. In the second in vivo approach a tissue-specific promoter, the 5kb GPX5 promoter, was used to direct expression of the large T-antigen to the epididymal duct epithelium.
KW - Epididymal
KW - Immortalization
KW - T-antigen
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U2 - 10.1016/j.mce.2003.10.073
DO - 10.1016/j.mce.2003.10.073
M3 - Review article
C2 - 15109748
AN - SCOPUS:11144356504
VL - 216
SP - 83
EP - 94
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1
ER -