Immobilized metal affinity chromatography in the presence of arginine

Ryota Abe, Motonori Kudou, Yoshikazu Tanaka, Tsutomu Arakawa, Kouhei Tsumoto

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Arginine hydrochloride (ArgHCl) is a versatile solvent additive, as it suppresses protein aggregation. ArgHCl has been used for protein refolding and to solubilize proteins from loose inclusion bodies. Immobilized metal affinity chromatography (IMAC) is one of the most commonly used technologies for purification of recombinant proteins. Here we have evaluated compatibility of ArgHCl with IMAC purification for his-tag proteins. ArgHCl clearly interfered with protein binding to Ni-columns. Nevertheless, such interference was greatly reduced at ArgHCl concentration below 200 mM, demonstrating that IMAC purification can be done even in the presence of ArgHCl.

Original languageEnglish
Pages (from-to)306-310
Number of pages5
JournalBiochemical and biophysical research communications
Volume381
Issue number3
DOIs
Publication statusPublished - 2009 Apr 10
Externally publishedYes

Keywords

  • Arginine hydrochloride
  • His-tag
  • Immobilized metal affinity chromatography
  • Solvent

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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