Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors

Ioulia G. Mourzina, Tatsuo Yoshinobu, Yuri E. Ermolenko, Yuri G. Vlasov, Michael J. Schöning, Hiroshi Iwasaki

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Various methods for the immobilization of urease and butyrylcholinesterase on the insulator surface of a laser-scanned semiconductor transducer (LSST) have been tested and compared for the development of an enzyme-based light-addressable potentiometric sensor (LAPS). The method of preparing photocurable membranes on LAPS is presented, and a new type of enzyme LAPS with photocurable polymeric enzyme membranes has been elaborated. It was found that sensors prepared by means of covalent bonding and cross-linking with inactive protein (type SIII) and with photocurable membrane matrices (type SIV) are more prospective. The enzyme LAPSensors with photocurable membranes demonstrate a degree of sensitivity close to the theoretical value and working ranges of 6.3•10-5-1.1•10-2 and 1•10 -4-1•10-1∈mol∈L-1 urea for acrylamide and acrylate-based membrane matrices, respectively, and 2.5•10-4-2•10-1∈mol∈L-1 butyrylcholine for an acrylamide membrane matrix. It is shown that such sensors can be also used for the analysis of enzyme inhibitors.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalMicrochimica Acta
Volume144
Issue number1-3
DOIs
Publication statusPublished - 2004
Externally publishedYes

Keywords

  • Enzyme immobilisation
  • Enzyme inhibitor
  • Enzyme-LAPS
  • Light-addressable potentiometric sensor
  • Photocurable polymer

ASJC Scopus subject areas

  • Analytical Chemistry

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