Immobilization of proteins on solid substrate employing affinity tags for surface forces measurement

Yoshiki Nakata, Takahiro Yamaguchi, Yuichiro Shimzu, Takehiro Suzuki, Kazuya Morikawa, Masaya Fujita, Kazue Kurihara

Research output: Contribution to conferencePaperpeer-review

Abstract

Proteins play significant roles in living systems through their interactions. So it is important to understand the interactions between them. For studying protein interactions by the surface forces measurement, it is essential to immobilize them on substrates. We immobilized proteins on the substrates employing affinity tags and measured protein interactions using colloid probe atomic force microscopy. This time, the interactions of SigB/RsbW and Spo0A-DB/DNA were studied.

Original languageEnglish
Pages5434-5435
Number of pages2
Publication statusPublished - 2006
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period06/9/2006/9/22

Keywords

  • Affinity tag
  • Atomic force microscopy
  • Immobilization
  • Protein
  • Surface force

ASJC Scopus subject areas

  • Engineering(all)

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