Imaging of the cortical cytoskeleton of guinea pig outer hair cells using atomic force microscopy

Hiroshi Wada, Kei Kimura, Takashi Gomi, Michiko Sugawara, Yukio Katori, Seiji Kakehata, Katsuhisa Ikeda, Toshimitsu Kobayashi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Mammalian outer hair cells (OHCs) are known to respond to acoustical stimulation with elongation and contraction of the cells' cylindrical soma in vivo, and this motility is related to both the protein motors distributed along the OHC plasma membrane and the cytoskeleton beneath it. Therefore, the cytoskeleton seems to play an important role in the motility of the OHC. Recently, an atomic force microscope (AFM) was used to investigate the OHC cytoskeleton under physiological conditions. However, details were not made clear in that study. In this study, the ultrastructure of the cytoskeleton of fixed OHCs of guinea pigs, which were extracted with Triton X-100, was investigated using the AFM. As a result, the cortical cytoskeleton, which is formed by discrete oriented domains, was imaged, and circumferential filaments and cross-links were observed within the domain. Morphological change of the cytoskeleton of the OHC induced by diamide treatment was then examined using the AFM, and reduction of cross-links was observed. The examination indicates that the cortical cytoskeleton comprises circumferential actin filaments and spectrin cross-links.

Original languageEnglish
Pages (from-to)51-62
Number of pages12
JournalHearing Research
Volume187
Issue number1-2
DOIs
Publication statusPublished - 2004 Jan

Keywords

  • Actin filament
  • Atomic force microscope
  • Cortical lattice
  • Outer hair cell
  • Spectrin cross-link

ASJC Scopus subject areas

  • Sensory Systems

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