Imaging of immobilized enzyme spots by scanning chemiluminescence microscopy with electrophoretic injection

Yu Hirano, Yutaro Mitsumori, Daisuke Oyamatsu, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Scanning chemiluminescence microscopy (SCLM) with electrophoretic injection was developed and applied to visualize enzyme reactions localized in an enzyme microspot. The SCLM uses a tapered glass capillary as a probe for injecting a small amount of luminol onto the substrate to generate localized chemiluminescence. The electrophoretic injection by application of a constant current between the inside and outside of the capillary enabled the continuous and controllable injection of a minute quantity of luminol in the range of 0.1 pmol/s. The image of enzyme activity in a monolayer spot of horseradish peroxidase was obtained by using the electrophoretic injection-based SCLM system.

Original languageEnglish
Pages (from-to)587-590
Number of pages4
JournalBiosensors and Bioelectronics
Volume18
Issue number5-6
DOIs
Publication statusPublished - 2003 May 1

Keywords

  • Electrophoretic injection
  • Imaging
  • Luminol
  • Microinjection
  • Scanning chemiluminescence microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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