Imaging of enzyme activity using bio-LSI system enables simultaneous immunosensing of different analytes in multiple specimens

Toshiki Hokuto, Tomoyuki Yasukawa, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Fumio Mizutani

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Electrochemical imaging is an excellent technique to characterize an activity of biomaterials, such as enzymes and cells. Large scale integration-based amperometric sensor (Bio-LSI) has been developed for the simultaneous and continuous detection of the concentration distribution of redox species generated by reactions of biomolecules. In this study, the Bio-LSI system was demonstrated to be applicable for simultaneous detection of different anaytes in multiple specimens. The multiple specimens containing human immunoglobulin G (hIgG) and mouse IgG (mIgG) were introduced into each channel of the upper substrate across the antibody lines for hIgG and mIgG on the lower substrate. Hydrogen peroxide generated by the enzyme reaction of glucose oxidase captured at intersections was simultaneously detected by 400 microelectrodes of Bio-LSI chip. The oxidation current increased with increasing the concentrations of hIgG, which can be detected in the range of 0.01–1.0 µg mL-1. Simultaneous detection of hIgG and mIgG in multiple specimens was achieved by using line pattern of both antibodies. Therefore, the presence of different target molecules in the multiple samples would be quantitatively and simultaneously visualized as a current image by the Bio-LSI system.

Original languageEnglish
Pages (from-to)838-842
Number of pages5
JournalBiotechnology Journal
Volume11
Issue number6
DOIs
Publication statusPublished - 2016 Jun 1

Keywords

  • Amperometric sensor
  • Electrochemical biosensing
  • Enzyme immunoassay
  • Multiple specimens
  • Simultaneous imaging

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

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