TY - JOUR
T1 - Imaging of enzyme activity by scanning electrochemical microscope equipped with a feedback control for substrate-probe distance
AU - Oyamatsu, Daisuke
AU - Hirano, Yu
AU - Kanaya, Norihiro
AU - Mase, Yoshiaki
AU - Nishizawa, Matsuhiko
AU - Matsue, Tomokazu
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Priority Area 417 (No. 14050010) and a Grant-in-Aid for the Creation of Innovations through Business–Academic–Public Sector Cooperation (No. 13504) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2003/8
Y1 - 2003/8
N2 - The enzymatic activity of diaphorase (Dp) immobilized on a solid substrate was characterized using a scanning electrochemical microscope (SECM) with shear force feedback to control the substrate-probe distance. The shear force between the substrate and the probe was monitored with a tuning fork-type quartz crystal and used as the feedback control to set the microelectrode probe close to the substrate surface. The sensitivity and the contrast of the SECM image were improved in the constant distance mode (distance, 50 nm) with the shear force feedback compared to the image in the constant height mode without the feedback. By using this system, the SECM and topographic images of the immobilized diaphorase were simultaneously measured. The microelectrode tip used in this study was ground aslant like a syringe needle in order to obtain the shaper topographic images. This shape was also effective for avoiding the interference during the diffusion of the enzyme substrates.
AB - The enzymatic activity of diaphorase (Dp) immobilized on a solid substrate was characterized using a scanning electrochemical microscope (SECM) with shear force feedback to control the substrate-probe distance. The shear force between the substrate and the probe was monitored with a tuning fork-type quartz crystal and used as the feedback control to set the microelectrode probe close to the substrate surface. The sensitivity and the contrast of the SECM image were improved in the constant distance mode (distance, 50 nm) with the shear force feedback compared to the image in the constant height mode without the feedback. By using this system, the SECM and topographic images of the immobilized diaphorase were simultaneously measured. The microelectrode tip used in this study was ground aslant like a syringe needle in order to obtain the shaper topographic images. This shape was also effective for avoiding the interference during the diffusion of the enzyme substrates.
KW - Diaphorase
KW - Feedback control
KW - SECM
KW - Shear force
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U2 - 10.1016/S1567-5394(03)00055-0
DO - 10.1016/S1567-5394(03)00055-0
M3 - Article
C2 - 12893317
AN - SCOPUS:0042170300
VL - 60
SP - 115
EP - 121
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
SN - 1567-5394
IS - 1-2
ER -