Imaging of EdU, an alkyne-tagged cell proliferation probe, by Raman microscopy

Hiroyuki Yamakoshi, Kosuke Dodo, Masaya Okada, Jun Ando, Almar Palonpon, Katsumasa Fujita, Satoshi Kawata, Mikiko Sodeoka

Research output: Contribution to journalArticlepeer-review

254 Citations (Scopus)


Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.

Original languageEnglish
Pages (from-to)6102-6105
Number of pages4
JournalJournal of the American Chemical Society
Issue number16
Publication statusPublished - 2011 Apr 27
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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