Imaging of astrocytic activity in living rodents

Norio Takata, Yoshiaki Shinohara, Masamichi Ohkura, Tsuneko Mishima, Junichi Nakai, Hajime Hirase

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca2+) concentration has been suggested to be important for gliotransmission; however, the study of Ca2+ dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca2+ concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron-glia interactions in the brain of living rodents.

Original languageEnglish
Title of host publicationOptical Imaging of Neocortical Dynamics
PublisherHumana Press Inc.
Pages191-207
Number of pages17
ISBN (Print)9781627037846
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameNeuromethods
Volume85
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Adenovirus
  • Astrocytes
  • Astrocytic processes
  • Bolus loading
  • Cerebral cortex
  • G-CaMP

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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