TY - JOUR
T1 - Imaging and treatment of malignant metastatic tumors by using radiation-sensitive, immunolabeled liquid-core microcapsules
AU - Harada, Satoshi
AU - Ehara, Shigeru
AU - Ishii, Keizo
AU - Sato, Takahiro
AU - Kouka, Masashi
AU - Kamiya, Tomihiro
AU - Sera, Koichiro
AU - Goto, Shyoko
PY - 2014
Y1 - 2014
N2 - In this study, two types of microcapsules were designed: (1) computed tomography (CT)-detectable anti-αvβ3 (E[c(RGDfK)]2) microcapsules, containing P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1), for the observation of metastases through αvβ3-antigen- antibody accumulation; and (2) metastasis-targeting microcapsules that upon irradiation release anticancer drugs with high affinity for P-selectin. These microcapsules were tested on C3He/N mice with MM48 tumors undergoing two radiotherapy sessions. The injected anti-αvβ3 microcapsules accumulated in the vascular endothelium of metastatic tissues and could be detected by CT. These microcapsules released P-selectin in response to the first irradiation, which was also induced in the endothelium of vessels in the case of metastasis. The microcapsules used for radiosensitizing metastases were injected 6 h after the first radiotherapy session. Their accumulation reached a maximum at 3 h after injection. After the second radiotherapy session, the microcapsules released carboplatin, which reduced the number of metastases; however, this reduction was not significant.
AB - In this study, two types of microcapsules were designed: (1) computed tomography (CT)-detectable anti-αvβ3 (E[c(RGDfK)]2) microcapsules, containing P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1), for the observation of metastases through αvβ3-antigen- antibody accumulation; and (2) metastasis-targeting microcapsules that upon irradiation release anticancer drugs with high affinity for P-selectin. These microcapsules were tested on C3He/N mice with MM48 tumors undergoing two radiotherapy sessions. The injected anti-αvβ3 microcapsules accumulated in the vascular endothelium of metastatic tissues and could be detected by CT. These microcapsules released P-selectin in response to the first irradiation, which was also induced in the endothelium of vessels in the case of metastasis. The microcapsules used for radiosensitizing metastases were injected 6 h after the first radiotherapy session. Their accumulation reached a maximum at 3 h after injection. After the second radiotherapy session, the microcapsules released carboplatin, which reduced the number of metastases; however, this reduction was not significant.
KW - Cancer
KW - Chemoradiotherapy
KW - Computed tomography
KW - Micro-PIXE camera
KW - Microcapsule imaging
KW - PIXE
UR - http://www.scopus.com/inward/record.url?scp=84888877389&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888877389&partnerID=8YFLogxK
U2 - 10.1016/j.nimb.2013.06.045
DO - 10.1016/j.nimb.2013.06.045
M3 - Article
AN - SCOPUS:84888877389
VL - 318
SP - 173
EP - 177
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
SN - 0168-583X
IS - PART A
ER -