IL-37 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption in vivo

Jafari Saeed, Hideki Kitaura, Keisuke Kimura, Masahiko Ishida, Haruki Sugisawa, Yumiko Ochi, Akiko Kishikawa, Teruko Yamamoto

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16 Citations (Scopus)

Abstract

IL-37 is a newly defined member of the IL-1 cytokine family. It has been reported that IL-37 inhibited innate immunity and inflammatory responses in autoimmune diseases and tumors. IL-37 also inhibited Lipopolysaccharide (LPS)-induced immunological reaction. LPS is a bacterial cell wall component that is capable of inducing osteoclast formation and pathological bone resorption. However, there is no study to investigate the effect of IL-37 on LPS-induced osteoclast formation and bone resorption. The purpose of this study is to investigate the effect of IL-37 in LPS-induced osteoclast formation and bone resorption.LPS was administrated with or without IL-37 by subcutaneous injection on mice calvariae. The number of osteoclasts, the level of tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA, the ratio of the bone resorption pits and the level of C-terminal telopeptide fragments of type I collagen cross-Links as a marker of bone resorption in mice administrated both LPS and IL-37 were lower than that in mice administrated LPS alone.Real-time RT-PCR was performed to analyze osteoclast related cytokines RANKL, TNF-α and IL-1β mRNA levels in vivo. RANKL, TNF-α and IL-1β mRNAs were increased in the LPS alone administrated mice as compared with PBS administrated groups. On the other hand, RANKL, TNF-α and IL-1β mRNAs were inhibited in the IL-37 and LPS administrated mice as compared with LPS alone administrated group.In vitro analysis, there was no effect of IL-37 in RANKL-induced osteoclast formation, TNF-α-induced osteoclast formation and cell viability from bone marrow macrophages as osteoclast precursor and LPS-induced RANKL expression from stromal cells.These results indicated that IL-37 inhibited LPS-induced osteoclast formation and bone resorption via inhibition of LPS-induced osteoclast related cytokines, but might not directly inhibit osteoclast formation on osteoclast precursor and RANKL expression on stromal cells.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalImmunology Letters
Volume175
DOIs
Publication statusPublished - 2016 Jul 1

Keywords

  • Cytokine
  • IL-37
  • LPS
  • Osteoclast

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Saeed, J., Kitaura, H., Kimura, K., Ishida, M., Sugisawa, H., Ochi, Y., Kishikawa, A., & Yamamoto, T. (2016). IL-37 inhibits lipopolysaccharide-induced osteoclast formation and bone resorption in vivo. Immunology Letters, 175, 8-15. https://doi.org/10.1016/j.imlet.2016.04.004