IL-2 receptor γchain expression on CD34 positive hematopoietic progenitor cells from bone marrow and cord blood

Masayuki Itano, Shigeru Tsuchiya, Sei Morita, Hiromi Fujie, Naoto Ishii, Takaaki Yanagisawa, Yoshiyuki Ohashi, Masayoshi Minegishi, Kazuo Sugamura, Tasuke Konno

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6 Citations (Scopus)

Abstract

The IL-2 receptor (IL-2R) γchain is shared among receptors for IL-4, IL-7, IL-9 and IL-15 as well as IL-2. In order to clarify the functional role of these cytokines interacting with the common γchain in human early hematopoiesis, we studied expression of the IL-2R γchain on purified CD34 positive cells from bone marrow and cord blood. Broad populations of bone marrow mononuclear cells were all found to express the IL-2R γchain. CD34 positive cells were purified by CD34 monoclonal antibodies and immunomagnetic beads as representative hematopoietic progenitor cells. It was established that only 38±10% of CD34 positive bone marrow cells (n=5) and 35±12% of CD34 positive cord blood cells (n=11) expressed the IL-2R γchain. CD34(+) IL-2R γchain(+) and CD34(+) IL-2R γchain(-) cells fractionated by cell sorting were subjected to clonogenic assays that showed granulocyte-macrophage colony-forming cells (CFU-GM) were present evenly in both fractions, whereas erythroid burst-forming cells (BFU-E) were enriched in the CD34(+) IL-2R γchain(-) fraction approximately two- to six-fold as compared with CD34(+) IL-2R γchain(+) fraction. Such clonogenic features did not differ between the bone marrow and cord blood cases. These results indicate that CD34(+) IL-2R γchain(-) cells contain immature cells already committed to the erythroid lineage.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalTohoku Journal of Experimental Medicine
Volume178
Issue number4
DOIs
Publication statusPublished - 1996 Apr

Keywords

  • BFU-E
  • CD34 positive cells
  • CFU-GM
  • IL-2 receptor γchain

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Itano, M., Tsuchiya, S., Morita, S., Fujie, H., Ishii, N., Yanagisawa, T., Ohashi, Y., Minegishi, M., Sugamura, K., & Konno, T. (1996). IL-2 receptor γchain expression on CD34 positive hematopoietic progenitor cells from bone marrow and cord blood. Tohoku Journal of Experimental Medicine, 178(4), 389-398. https://doi.org/10.1620/tjem.178.389