IL-17A promotes the growth of airway epithelial cells through ERK-dependent signaling pathway

Daisuke Inoue, Muneo Numasaki, Mika Watanabe, Hiroshi Kubo, Takahiko Sasaki, Hiroyasu Yasuda, Mutsuo Yamaya, Hidetada Sasaki

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

The effects of IL-17A on mucin production and growth of airway epithelial cells were examined. Histological and immunohistochemical analyses revealed that IL-17A increased the mucin production and number of tracheal epithelial cells in air-liquid interface cultures. The biological property of IL-17A to stimulate the mucin production by tracheal epithelial cells was determined using an ELISA. The mitogenic effect of IL-17A on tracheal epithelial cells was confirmed with Calcein-AM assay. The growth-stimulatory effect of IL-17A was dose-dependent and mediated via the ERK MAP kinase pathway. Inhibitors of MEK abrogated the mitogenic effect of IL-17A, whereas an inhibitor of p38 or JNK displayed no significant inhibitory effect. Moreover, relatively lower doses of IL-13 also significantly increased the growth of tracheal epithelial cells through a distinct signaling pathway from that of IL-17A. These findings provide the first evidence that IL-17A stimulates the growth of airway epithelial cells through the ERK MAP kinase pathway.

Original languageEnglish
Pages (from-to)852-858
Number of pages7
JournalBiochemical and biophysical research communications
Volume347
Issue number4
DOIs
Publication statusPublished - 2006 Sep 8

Keywords

  • Airway epithelial cells, and mitogen-activated protein kinase kinase
  • IL-17A
  • Mucin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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