IL-12p40 gene expression in lung and hilar lymph nodes of MPS-resistant pigs

Akiko Sakuma, Shizuka Sugawara, Hikaru Hidaka, Mitsuru Nakajo, Yoshihito Suda, Tomoyuki Shimazu, Michael T. Rose, Megumi Urakawa, Tao Zhuang, Guoqi Zhao, Kouichi Watanabe, Tomonori Nochi, Haruki Kitazawa, Kazuo Katoh, Keiichi Suzuki, Hisashi Aso

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Mycoplasma pneumonia of swine (MPS) is caused by Mycoplasma hyopneumoniae (M.hp) and is a common chronic respiratory disease of pigs. Recently, a genetically selected variant of the Landrace pig (Miyagino L2) has a lower incidence of pulmonary MPS lesions. We investigated the pathological and immunological characteristics of MPS resistance in these pigs (n = 24) by comparing with the normal landrace pig (control: n = 24). The pathological MPS lung lesion score in MPS-selected landrace pigs was significantly lower than in the control. The gene expression of interleukin (IL)-12p40, which acts as a chemoattractant and a component of the bioactive cytokines IL-12 and IL-23, was significantly higher at the hilar lymph nodes, lung, and spleen in MPS-selected landrace pigs than in control landrace pigs, and these were negatively correlated with the macroscopic MPS lung lesion score. In summary, we demonstrate that resistance against MPS in Miyagino L2 pigs is associated with IL-12p40 up-regulation, in comparison with normal landrace pigs without the MPS vaccine. In addition, a comparative study of macroscopic MPS lung lesions and IL-12p40 gene expression in lung and hilar lymph nodes may lead to beneficial selection traits for the genetic selection for MPS resistance in pigs.

    Original languageEnglish
    Article numbere13450
    JournalAnimal Science Journal
    Volume91
    Issue number1
    DOIs
    Publication statusPublished - 2020 Jan 1

    Keywords

    • Mycoplasma hyopneumoniae
    • cytokine
    • disease resistance
    • histopathology
    • landrace pigs

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)

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