IL-12-mediated and IL-18-mediated Nitric oxide-induced apoptosis of adherent bone marrow cells in TNF-α-induced osteoclast formation

Hideki Kitaura, Tomo Aonuma, Emiko Fukumoto, Keisuke Kimura, Toshiya Fujii, Zaki Weli Hakami, Teruko Takano-Yamamoto

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

It has recently been reported that TNF-a has the ability to accelerate osteoclastogenesis. We previously reported that the proinflammatory cytokine IL-12 and IL-18 induced apoptosis in TNF-α-mediated osteoclastogenesis in mouse bone marrow culture through an interaction of Fas and Fas ligand (FasL). However, the inhibition of Fas-FasL interaction by using anti-FasL antibody could not completely inhibit apoptosis. Therefore, it is possible that IL-12 and IL-18 may also trigger some other apoptotic mechanisms. Nitric oxide (NO) may act as a mediator of the apoptotic effect. We found that NO production was induced in bone marrow cells cultured with IL-12 and IL-18 in the presence of TNF-α. Apoptosis was partially inhibited when bone marrow cells were treated with NO inhibitors. The simultane- ous effects of TNF-α and IL-12 or IL-18 on bone marrow cells induce apoptosis by the production of NO.

Original languageEnglish
Title of host publicationInterface Oral Health Science 2011
PublisherSpringer Japan
Pages125-127
Number of pages3
ISBN (Electronic)9784431540700
ISBN (Print)9784431540694
DOIs
Publication statusPublished - 2012 Jan 1

Keywords

  • Apoptosis
  • IL-12
  • IL-18
  • Nitric oxide
  • Osteoclast
  • TNF-α

ASJC Scopus subject areas

  • Dentistry(all)

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