IL-1β and IL-6 in mouse parotid acinar cells: Characterization of synthesis, storage, and release

Naoko Tanda, Hiroe Ohyama, Midori Yamakawa, Maria Ericsson, Takanori Tsuji, Jim McBride, Aram Elovic, David T.W. Wong, Gary R. Login

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Synthesis, storage, and secretion of the proinflammatory cytokine interleukin-1β (IL-1β) and the anti-inflAmmatory cytokine IL-6 have not been established in normal exocrine gland secretory cells. Parotid glands and isolated acinar cells prepared from BALB/c mice were homogenized for RNA isolation and reverse transcription-polymerase chain reaction (RT-PCR). IL- 1β and IL-6 enzyme-linked immunosorbent assays (ELISAs) were done on supernatants prepared from mouse parotid acinar cell (MPAC) preparations unstimulated or stimulated between 0 and 10 rain with 10-5 M norepinephrine at 37°C. MPACs were fixed in paraformaldehyde, frozen sectioned for light and electron microscopy, and labeled with antibodies to IL-1β and IL-6. Mouse specific riboprobes to IL-1 and IL-6 were used for in situ hybridization. RT-PCR yielded the expected IL-1 (336-bp) and IL-6 (614-bp) mRNA products. By ELISA, stimulated MPACs showed a significant increase in IL-1β (P < 0.03) and IL-6 (P < 0.01) release into supernatants by 10 min that paralleled the time course of amylase release. In situ hybridization showed the presence of transcripts for IL-1 and IL-6 in glandular epithelial cells. Gold-labeled IL-1β and IL-6 were significantly higher (P < 0.01) in granules than in the nucleus and cytoplasm. This study shows that MPACs synthesize IL-1β and IL-6 and release these cytokines from their granules after α- and β-adrenergic stimulation.

Original languageEnglish
Pages (from-to)G147-G156
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume274
Issue number1 37-1
DOIs
Publication statusPublished - 1998 Jan
Externally publishedYes

Keywords

  • Cytokines
  • Exocytosis
  • Immunoelectron microscopy
  • Parotid gland
  • Reverse transcription-polymerase chain reaction
  • Secretion

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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