IKKε Regulates F Actin Assembly and Interacts with Drosophila IAP1 in Cellular Morphogenesis

Kenzi Oshima, Michiko Takeda, Erina Kuranaga, Ryu Ueda, Toshiro Aigaki, Masayuki Miura, Shigeo Hayashi

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)


Differentiated cells assume complex shapes through polarized cell migration and growth. These processes require the restricted organization of the actin cytoskeleton at limited subcellular regions. IKKε is a member of the IκB kinase family, and its developmental role has not been clear. Drosophila IKKε was localized to the ruffling membrane of cultured cells and was required for F actin turnover at the cell margin. In IKKε mutants, tracheal terminal cells, bristles, and arista laterals, which require accurate F actin assembly for their polarized elongation, all exhibited aberrantly branched morphology. These phenotypes were sensitive to a change in the dosage of Drosophila inhibitor of apoptosis protein 1 (DIAP1) and the caspase DRONC without apparent change in cell viability. In contrast to this, hyperactivation of IKKε destabilized F actin-based structures. Expression of a dominant-negative form of IKKε increased the amount of DIAP1. The results suggest that at the physiological level, IKKε acts as a negative regulator of F actin assembly and maintains the fidelity of polarized elongation during cell morphogenesis. This IKKε function involves the negative regulation of the nonapoptotic activity of DIAP1.

Original languageEnglish
Pages (from-to)1531-1537
Number of pages7
JournalCurrent Biology
Issue number15
Publication statusPublished - 2006 Aug 8
Externally publishedYes



ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)


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