IgE antibody to fish gelatin (type I collagen) in patients with fish allergy

Masahiro Sakaguchi, Masako Toda, Testuya Ebihara, Shinkichi Irie, Hisae Hori, Atsushi Imai, Makoto Yanagida, Hiroshi Miyazawa, Hiroyuki Ohsuna, Zenro Ikezawa, Sakae Inouye

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)

Abstract

Background: Most children with anaphylaxis to measles, mumps, and rubella vaccines had shown sensitivity to bovine gelatin that was included in the vaccines. Recently, it was found that bovine type I collagen, which is the main content in the gelatin, is a major allergen in bovine gelatin allergy. Fish meat and skin also contain type I collagen. Objective: The present study was designed to investigate IgE antibody to fish gelatin in children with fish allergy. Methods: Serum samples were taken from patients in 3 groups: (1) 10 patients with fish allergy and specific IgE to fish meat; (2) two patients with allergies to both fish meat and bovine gelatin and specific IgE to fish meat and bovine gelatin; and (3) 15 patients with atopic dermatitis and specific IgE to fish meat. Various fish gelatins (type I collagen) were prepared from fish skin. IgE antibody to fish gelatin was analyzed by using ELISA and immunoblotting. Results: Of 10 patients with fish allergy, 3 had specific IgE to fish gelatin. Of two patients with fish allergy and bovine gelatin allergy, all had specific IgE to fish gelatin. Of 15 patients with atopic dermatitis and specific IgE to fish meat, 5 had specific IgE to fish gelatin. Furthermore, IgE from pooled serum of the patients reacted with both the α1 and α2 chains of fish type I collagen in immunoblots. There is cross-reactivity among gelatins from various fishes, but there is little cross-reactivity between fish and bovine gelatins. Conclusion: Some fish-sensitive patients possessed IgE antibody to fish gelatin. Fish gelatin (type I collagen) might be an allergen in subjects with fish allergy.

Original languageEnglish
Pages (from-to)579-584
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Volume106
Issue number3
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Allergen
  • Fish allergy
  • Gelatin
  • IgE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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