Identification of two common regions of allelic loss in chromosome arm 12q in human pancreatic cancer

Mitsuhiro Kimura, Toru Furukawa, Tadayoshi Abe, Toshimasa Yatsuoka, Emile M. Youssef, Tadaaki Yokoyama, Hong Ouyang, Yasuyuki Ohnishi, Makoto Sunamura, Masao Kobari, Seiki Matsuno, Akira Horii

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Using the method of microsatellite analysis, we studied 40 tissues with pancreatic ductal adenocarcinoma and identified two commonly deleted regions on the long arm of chromosome 12. One (region A) was found between D12S81 and D12S1719 at 12q21 at a frequency of 67.5%, and the other (region B) was located between D12S360 and D12S78 at 12q22-q23.1 at a frequency of 60%; the latter was reported previously (M. Kimura, et al. Genes Chromosomes Cancer, 17: 88-93, 1996). The results of microsatellite analyses were verified by fluorescence in situ hybridization. We further analyzed 19 pancreatic cancer cell lines by fluorescence in situ hybridization and found that 10 of them showed allelic loss at D12S81 and 6 showed allelic loss at D12S360. Yeast artificial chromosome contigs were constructed to cover the deleted regions. Region B was completely covered by a 650-kb yeast artificial chromosome clone. The frequently deleted regions in chromosome 12q in pancreatic cancer that were identified here may provide new avenues for isolating novel tumor suppressor genes.

Original languageEnglish
Pages (from-to)2456-2460
Number of pages5
JournalCancer Research
Volume58
Issue number11
Publication statusPublished - 1998 Jun 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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