Identification of two α-subunit species of GTP-binding proteins, Gα15 and Gαq, expressed in rat taste buds

Yuko Kusakabe, Eiri Yamaguchi, Kentaro Tanemura, Kimihiko Kameyama, Noboru Chiba, Soichi Arai, Yasufumi Emori, Keiko Abe

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

We cloned cDNAs for two G protein α-subunits belonging to the Gαq family, each capable of activating PLCβ, from rat tongue. One is a Gαq in the narrow sense, and the other, termed rat Gα15, is a rat counterpart of mouse Gα15, sharing an amino acid sequence similarity of 94%. RT-PCR and Northern blot analysis demonstrated that rat Gα15 and Gαq were distinctly expressed in tongue epithelia containing taste buds. Immunostaining also showed that rat Gα15, together with the Gαq, was localized mainly in taste buds. These studies suggest the possibility that these two Gα proteins function for taste signal transduction in sensory cells. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1403
Issue number3
DOIs
Publication statusPublished - 1998 Jul 24

Keywords

  • G protein
  • Gαq family
  • PLCβ
  • Taste bud
  • Taste signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Identification of two α-subunit species of GTP-binding proteins, Gα15 and Gαq, expressed in rat taste buds'. Together they form a unique fingerprint.

Cite this