Identification of TROSPA homologue in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan

Satoru Konnai; Shinji Yamada; Saiki Imamura; Hideto Nishikado; Naftaly Githaka; Takuya Ito; Ai Takano; Hiroki Kawabata; Shiro Murata; Kazuhiko Ohashi

doi:10.1016/j.ttbdis.2012.02.001

Identification of TROSPA homologue in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan

Satoru Konnai, Shinji Yamada, Saiki Imamura, Hideto Nishikado, Naftaly Githaka, Takuya Ito, Ai Takano, Hiroki Kawabata, Shiro Murata, Kazuhiko Ohashi

Medicine

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The tick receptor for outer surface protein A (TROSPA) is an . Ixodes scapularis (. I. scapularis) receptor for . Borrelia burgdorferi (. B. burgdorferi), the causative agent of Lyme disease in North America. The blockade of TROSPA has been shown to reduce . B. burgdorferi adherence to the . I. scapularis gut in vivo. Thus, TROSPA is one of the potential targets for the development of vector-antigen-based vaccines to prevent the transmission of . B. burgdorferi. The aim of this study is to identify the TROSPA gene in . I. persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. The cDNA clone encoding the TROSPA-like sequence with 483 nucleotides was obtained from whole-body homogenates of fed nymphs of . I. persulcatus. The putative amino acid sequence of . I. persulcatus TROSPA was 88.2% and 87.8% identical to that of . I. scapularis and . I. ricinus, respectively. This finding will facilitate investigations on the role of . I. persulcatus TROSPA and its interaction with . Borrelia spp. and will have important implications on endeavors to develop a tick vaccine.

Original language	English
Pages (from-to)	75-77
Number of pages	3
Journal	Ticks and Tick-borne Diseases
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.ttbdis.2012.02.001
Publication status	Published - 2012 Apr

Keywords

Borrelia
I. persulcatus
Lyme disease
TROSPA
Tick receptor

ASJC Scopus subject areas

Parasitology
Microbiology
Insect Science
Infectious Diseases

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Identification of TROSPA homologue in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. / Konnai, Satoru; Yamada, Shinji; Imamura, Saiki; Nishikado, Hideto; Githaka, Naftaly; Ito, Takuya; Takano, Ai; Kawabata, Hiroki; Murata, Shiro; Ohashi, Kazuhiko.

In: Ticks and Tick-borne Diseases, Vol. 3, No. 2, 04.2012, p. 75-77.

Research output: Contribution to journal › Article › peer-review

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title = "Identification of TROSPA homologue in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan",

abstract = "The tick receptor for outer surface protein A (TROSPA) is an . Ixodes scapularis (. I. scapularis) receptor for . Borrelia burgdorferi (. B. burgdorferi), the causative agent of Lyme disease in North America. The blockade of TROSPA has been shown to reduce . B. burgdorferi adherence to the . I. scapularis gut in vivo. Thus, TROSPA is one of the potential targets for the development of vector-antigen-based vaccines to prevent the transmission of . B. burgdorferi. The aim of this study is to identify the TROSPA gene in . I. persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. The cDNA clone encoding the TROSPA-like sequence with 483 nucleotides was obtained from whole-body homogenates of fed nymphs of . I. persulcatus. The putative amino acid sequence of . I. persulcatus TROSPA was 88.2% and 87.8% identical to that of . I. scapularis and . I. ricinus, respectively. This finding will facilitate investigations on the role of . I. persulcatus TROSPA and its interaction with . Borrelia spp. and will have important implications on endeavors to develop a tick vaccine.",

keywords = "Borrelia, I. persulcatus, Lyme disease, TROSPA, Tick receptor",

author = "Satoru Konnai and Shinji Yamada and Saiki Imamura and Hideto Nishikado and Naftaly Githaka and Takuya Ito and Ai Takano and Hiroki Kawabata and Shiro Murata and Kazuhiko Ohashi",

note = "Funding Information: This work was supported by grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and by a special grant for the promotion of research from the Global COE Program for Zoonosis Control, Hokkaido University . Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

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AU - Yamada, Shinji

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AU - Nishikado, Hideto

AU - Githaka, Naftaly

AU - Ito, Takuya

AU - Takano, Ai

AU - Kawabata, Hiroki

AU - Murata, Shiro

AU - Ohashi, Kazuhiko

N1 - Funding Information: This work was supported by grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and by a special grant for the promotion of research from the Global COE Program for Zoonosis Control, Hokkaido University . Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/4

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N2 - The tick receptor for outer surface protein A (TROSPA) is an . Ixodes scapularis (. I. scapularis) receptor for . Borrelia burgdorferi (. B. burgdorferi), the causative agent of Lyme disease in North America. The blockade of TROSPA has been shown to reduce . B. burgdorferi adherence to the . I. scapularis gut in vivo. Thus, TROSPA is one of the potential targets for the development of vector-antigen-based vaccines to prevent the transmission of . B. burgdorferi. The aim of this study is to identify the TROSPA gene in . I. persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. The cDNA clone encoding the TROSPA-like sequence with 483 nucleotides was obtained from whole-body homogenates of fed nymphs of . I. persulcatus. The putative amino acid sequence of . I. persulcatus TROSPA was 88.2% and 87.8% identical to that of . I. scapularis and . I. ricinus, respectively. This finding will facilitate investigations on the role of . I. persulcatus TROSPA and its interaction with . Borrelia spp. and will have important implications on endeavors to develop a tick vaccine.

AB - The tick receptor for outer surface protein A (TROSPA) is an . Ixodes scapularis (. I. scapularis) receptor for . Borrelia burgdorferi (. B. burgdorferi), the causative agent of Lyme disease in North America. The blockade of TROSPA has been shown to reduce . B. burgdorferi adherence to the . I. scapularis gut in vivo. Thus, TROSPA is one of the potential targets for the development of vector-antigen-based vaccines to prevent the transmission of . B. burgdorferi. The aim of this study is to identify the TROSPA gene in . I. persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. The cDNA clone encoding the TROSPA-like sequence with 483 nucleotides was obtained from whole-body homogenates of fed nymphs of . I. persulcatus. The putative amino acid sequence of . I. persulcatus TROSPA was 88.2% and 87.8% identical to that of . I. scapularis and . I. ricinus, respectively. This finding will facilitate investigations on the role of . I. persulcatus TROSPA and its interaction with . Borrelia spp. and will have important implications on endeavors to develop a tick vaccine.

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