Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital

Kouichi Yoshinari, Kaoru Kobayashi, Rick Moore, Takeshi Kawamoto, Masahiko Negishi

Research output: Contribution to journalArticlepeer-review

136 Citations (Scopus)

Abstract

The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.

Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalFEBS Letters
Volume548
Issue number1-3
DOIs
Publication statusPublished - 2003 Jul 31

Keywords

  • Heat shock protein
  • Nuclear receptor CAR
  • Nuclear translocation
  • Phenobarbital
  • Protein phosphatase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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