Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital

Kouichi Yoshinari; Kaoru Kobayashi; Rick Moore; Takeshi Kawamoto; Masahiko Negishi

doi:10.1016/S0014-5793(03)00720-8

Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital

Kouichi Yoshinari, Kaoru Kobayashi, Rick Moore, Takeshi Kawamoto, Masahiko Negishi

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

137 Citations (Scopus)

Abstract

The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.

Original language	English
Pages (from-to)	17-20
Number of pages	4
Journal	FEBS Letters
Volume	548
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00720-8
Publication status	Published - 2003 Jul 31

Keywords

Heat shock protein
Nuclear receptor CAR
Nuclear translocation
Phenobarbital
Protein phosphatase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)00720-8

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title = "Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital",

abstract = "The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.",

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T1 - Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital

AU - Yoshinari, Kouichi

AU - Kobayashi, Kaoru

AU - Moore, Rick

AU - Kawamoto, Takeshi

AU - Negishi, Masahiko

PY - 2003/7/31

Y1 - 2003/7/31

N2 - The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.

AB - The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.

KW - Heat shock protein

KW - Nuclear receptor CAR

KW - Nuclear translocation

KW - Phenobarbital

KW - Protein phosphatase

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Identification of the nuclear receptor CAR:HSP90 complex in mouse liver and recruitment of protein phosphatase 2A in response to phenobarbital

Abstract

Keywords

ASJC Scopus subject areas

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