Identification of the multifunctional calmodulin-dependent protein kinase in the cytosol, sarcoplasmic reticulum, and sarcolemma of rabbit skeletal muscle

Hiroshi Sato; Kohji Fukunaga; Shukuro Araki; Iwao Ohtsuki; Eishichi Miyamoto

doi:10.1016/0003-9861(88)90468-7

Identification of the multifunctional calmodulin-dependent protein kinase in the cytosol, sarcoplasmic reticulum, and sarcolemma of rabbit skeletal muscle

Hiroshi Sato, Kohji Fukunaga, Shukuro Araki, Iwao Ohtsuki, Eishichi Miyamoto

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A multifunctional calmodulin-dependent protein kinase (calmodulin kinase) was purified from the cytosol of rabbit skeletal muscle as a subunit of 58 kDa. A 58-kDa protein in sarcoplasmic reticulum (SR) and sarcolemma (SL) of rabbit skeletal muscle was endogenously phosphorylated in a calmodulin-dependent manner. The 58-kDa protein in SR and SL was considered to be identical to the subunit of cytosol calmodulin kinase on the basis of immunoreactivity, calmodulin binding, and autophosphorylation studies and on the patterns of protease-treated phosphopeptides. Calmodulin kinase showed broad substrate specificity and phosphorylated troponins I and T.

Original language	English
Pages (from-to)	443-451
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	260
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(88)90468-7
Publication status	Published - 1988 Jan
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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Identification of the multifunctional calmodulin-dependent protein kinase in the cytosol, sarcoplasmic reticulum, and sarcolemma of rabbit skeletal muscle. / Sato, Hiroshi; Fukunaga, Kohji; Araki, Shukuro; Ohtsuki, Iwao; Miyamoto, Eishichi.

In: Archives of Biochemistry and Biophysics, Vol. 260, No. 1, 01.1988, p. 443-451.

Research output: Contribution to journal › Article › peer-review

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