Identification of the hydrogen uptake gene cluster for chemolithoautotrophic growth and symbiosis hydrogen uptake in Bradyrhizobium Diazoefficiens

Sachiko Masuda, Masaki Saito, Chiaki Sugawara, Manabu Itakura, Shima Eda, Kiwamu Minamisawa

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H2 released by nitrogenase in soybean nodule symbiosis, and is responsible for H2-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H2-dependent chemolithoautotrophic conditions was markedly weaker and H2 production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS1L1) than for the mutant of hup locus II (ΔhupS2L2). These results indicate that locus I is primarily responsible for Hup activity.

    Original languageEnglish
    Pages (from-to)76-78
    Number of pages3
    JournalMicrobes and environments
    Volume31
    Issue number1
    DOIs
    Publication statusPublished - 2016 Mar 26

    Keywords

    • Bradyrhizobium diazoefficiens
    • Chemolithoautotrophic growth
    • Hydrogenase
    • Symbiosis

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

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