Identification of the hydrogen uptake gene cluster for chemolithoautotrophic growth and symbiosis hydrogen uptake in Bradyrhizobium Diazoefficiens

Sachiko Masuda; Masaki Saito; Chiaki Sugawara; Manabu Itakura; Shima Eda; Kiwamu Minamisawa

doi:10.1264/jsme2.ME15182

Identification of the hydrogen uptake gene cluster for chemolithoautotrophic growth and symbiosis hydrogen uptake in Bradyrhizobium Diazoefficiens

Sachiko Masuda, Masaki Saito, Chiaki Sugawara, Manabu Itakura, Shima Eda, Kiwamu Minamisawa

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H₂ released by nitrogenase in soybean nodule symbiosis, and is responsible for H₂-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H₂-dependent chemolithoautotrophic conditions was markedly weaker and H2 production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS₁L₁) than for the mutant of hup locus II (ΔhupS₂L₂). These results indicate that locus I is primarily responsible for Hup activity.

Original language	English
Pages (from-to)	76-78
Number of pages	3
Journal	Microbes and environments
Volume	31
Issue number	1
DOIs	https://doi.org/10.1264/jsme2.ME15182
Publication status	Published - 2016 Mar 26

Keywords

Bradyrhizobium diazoefficiens
Chemolithoautotrophic growth
Hydrogenase
Symbiosis

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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10.1264/jsme2.ME15182

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Identification of the hydrogen uptake gene cluster for chemolithoautotrophic growth and symbiosis hydrogen uptake in Bradyrhizobium Diazoefficiens. / Masuda, Sachiko; Saito, Masaki; Sugawara, Chiaki; Itakura, Manabu; Eda, Shima; Minamisawa, Kiwamu.

In: Microbes and environments, Vol. 31, No. 1, 26.03.2016, p. 76-78.

Research output: Contribution to journal › Article › peer-review

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abstract = "The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H2 released by nitrogenase in soybean nodule symbiosis, and is responsible for H2-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H2-dependent chemolithoautotrophic conditions was markedly weaker and H2 production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS1L1) than for the mutant of hup locus II (ΔhupS2L2). These results indicate that locus I is primarily responsible for Hup activity.",

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AU - Itakura, Manabu

AU - Eda, Shima

AU - Minamisawa, Kiwamu

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